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Fractionation and bioaccessibility of manganese, copper, zinc, cadmium, and lead in commercial vegetable and rice baby foods using inductively coupled plasma-mass spectrometry (ICP-MS) with central composite design (CCD)

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2019-07-04

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Taylor & Francis Inc

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Abstract

Herein the bioaccessibility of Mn, Cu, Zn, Cd, and Pb, selected from essential and toxic elements, was determined in commercially sold vegetable purees intended for infant and toddler consumption. Chemical fractionation studies using water, acetone, diethyl ether, chloroform:methanol, and n-hexane were employed to predict the importance of the protein and lipid parts of a matrix to assess the bioaccessibility data. In addition, in-vitro gastrointestinal digestion was performed to determine the bioaccessibility of the elements using a five level, three factor central composite design (CCD) to maximize the elemental solubility. The total elemental concentrations in all of the fractions were determined by inductively coupled plasma - mass spectrometry (ICP-MS). Based on the consumption of one jar of vegetable/rice-based baby food, Zn was 1.3% of the recommended dietary allowance and Mn was 4.2% of the adequate intake level, while Cu was almost 100% of the adequate intake level. Additionally, Pb was always below the detection limit and Cd was sometimes under the detection limit for the percent bioaccessibility. However, in some samples, Cd was as high as 80% of the tolerable weekly intake level depending on the body weight.

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Camellia-sinensis l., Chemical fractionation, Bioavailability, Elements, Ingredients, Nutrition, Minerals, Sample, Soil, Mn, Baby food, Trace elements, Fractionation, Bioaccessibility, Inductively coupled plasma - mass spectrometry (icp-ms) with central composite design (ccd), Chemistry

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