Publication: Viral and atypical bacterial respiratory infections in a university teaching hospital
dc.contributor.author | Harun, Ağca | |
dc.contributor.author | Beyza, Ener | |
dc.contributor.buuauthor | AĞCA, HARUN | |
dc.contributor.buuauthor | ENER, BEYZA | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Mikrobiyoloji Anabilim Dalı | |
dc.contributor.orcid | 0000-0002-2651-2034 | |
dc.contributor.researcherid | AAH-4027-2021 | |
dc.contributor.researcherid | ISU-9626-2023 | |
dc.date.accessioned | 2024-07-16T06:12:50Z | |
dc.date.available | 2024-07-16T06:12:50Z | |
dc.date.issued | 2019-09-01 | |
dc.description.abstract | Respiratory viral and atypical bacterial agents lead to infections in a large spectrum, from mild symptoms to respiratory failure. In the present study, we aimed to detect multiple viral and bacterial agents in the respiratory samples of inpatients by real-time polymerase chain reaction (RT-PCR). Nasopharyngeal swabs and broncho-alveolar lavage samples from inpatients with respiratory infection symptoms at the Uludag University Hospital between December 1, 2015 and March 31,2018 were investigated. DNA/RNA was extracted using the EZ1 Virus Mini Kit v2.0 (Qiagen, Belgium) with the EZ1 extraction device (Qiagen, Belgium). The R-GENE (R) RT-PCR (Biomerioux, France) kit was used to detect influenza A, influenza B, respiratory syncytial virus (RSV), human metapneumovirus, rhinovirus/enterovirus (RV/EV), adenovirus, human bocavirus (hBoV), corona virus, parainfluenza virus, Chlamydia pneumoniae/Mycoplasma pneumoniae, and Legionella pneumophila in Rotor-Gene Q (Qiagen, Belgium). Patients were aged between 0 and 90 years. Overall, 177 (56.9%) patients were men and 134 (43.1%) were women. A total of 311 samples were analyzed, of which 214 (68.8%) were positive. In total, 360 agents, including 338 viruses and 22 bacteria, were detected. The commonest agents were influenza A+B (n = 65, 18,1%), hBoV (n = 64, 17.8%), RV/EV (n = 56, 15.6%), and RSV (n = 47, 13.1%). Rapid diagnosis of viral infections by RT-PCR is important for the specific treatment of patients. | |
dc.identifier.doi | 10.7883/yoken.JJID.2018.510 | |
dc.identifier.eissn | 1884-2836 | |
dc.identifier.endpage | 322 | |
dc.identifier.issn | 1344-6304 | |
dc.identifier.issue | 5 | |
dc.identifier.startpage | 318 | |
dc.identifier.uri | https://doi.org/10.7883/yoken.JJID.2018.510 | |
dc.identifier.uri | https://www.jstage.jst.go.jp/article/yoken/72/5/72_JJID.2018.510/_article | |
dc.identifier.uri | https://hdl.handle.net/11452/43277 | |
dc.identifier.volume | 72 | |
dc.identifier.wos | 000489677000006 | |
dc.indexed.wos | WOS.SCI | |
dc.language.iso | en | |
dc.publisher | Natl Inst Infectious Diseases | |
dc.relation.journal | Japanese Journal of Infectious Diseases | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Stem-cell transplantation | |
dc.subject | Real-time pcr | |
dc.subject | Enterovirus d68 | |
dc.subject | Human bocavirus | |
dc.subject | Rhinovirus | |
dc.subject | Pneumonia | |
dc.subject | Outpatients | |
dc.subject | Mortality | |
dc.subject | Influenza | |
dc.subject | Diagnosis | |
dc.subject | Science & technology | |
dc.subject | Life sciences & biomedicine | |
dc.subject | Infectious diseases | |
dc.title | Viral and atypical bacterial respiratory infections in a university teaching hospital | |
dc.type | Article | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | eeb102e3-a297-417f-962b-8b6991f5b89b | |
relation.isAuthorOfPublication | 2b082cc1-092b-441d-bafb-e08676bd66bb | |
relation.isAuthorOfPublication.latestForDiscovery | eeb102e3-a297-417f-962b-8b6991f5b89b |
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