Publication: Early-stage colon cancer with high malat1 expression is associated with the 5-fluorouracil resistance and future metastasis
dc.contributor.author | Öztürk, Ersin | |
dc.contributor.buuauthor | Aksoy, Seçil Ak | |
dc.contributor.buuauthor | Tunca, Berrin | |
dc.contributor.buuauthor | TUNCA, BERRİN | |
dc.contributor.buuauthor | Erçelik, Melis | |
dc.contributor.buuauthor | Tezcan, Gulcin | |
dc.contributor.buuauthor | TEZCAN, GÜLÇİN | |
dc.contributor.buuauthor | Çeçener, Gülşah | |
dc.contributor.buuauthor | ÇEÇENER, GÜLŞAH | |
dc.contributor.buuauthor | UĞRAŞ, NESRİN | |
dc.contributor.buuauthor | YILMAZLAR, AHMET TUNCAY | |
dc.contributor.buuauthor | Yerci, Omer | |
dc.contributor.buuauthor | YERCİ, ÖMER | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji Anabilim Dalı. | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı. | |
dc.contributor.orcid | 0000-0002-1619-6680 | |
dc.contributor.orcid | 0000-0002-5956-8755 | |
dc.contributor.orcid | 0000-0001-8593-5101 | |
dc.contributor.orcid | 0000-0002-3820-424X | |
dc.contributor.researcherid | ADM-8457-2022 | |
dc.contributor.researcherid | AAH-3843-2020 | |
dc.date.accessioned | 2024-09-13T06:03:09Z | |
dc.date.available | 2024-09-13T06:03:09Z | |
dc.date.issued | 2022-07-06 | |
dc.description.abstract | Background This study aimed to investigate the role of long noncoding RNA (LncRNA) expression profiles to predict relapse and 5-FU response in patients with stage I/II colon cancer (CC). Methods and Results The expression level of 15 LncRNA was analyzed in stage I/II colon tumors of 126 CC patients. To confirm the findings in-vitro, 5FU-resistant HT29 cells were generated by subjecting HT-29 cells to the increasing concentrations of 5FU for 6 months. The 5FU resistance was observed in WST-1 and Annexin V analyses. The colony formation and wound healing assays were assessed to determine the metastatic properties of the cells. Expression levels of LncRNAs and mRNA of EMT-related genes were determined by RT-PCR. The role of LncRNA on metastasis and 5FU sensitivity were confirmed in pcDNA3.0-PTENP1 and si-MALAT1 expressed 5FU-resistant HT29 cell lineages. Results High MALAT1 (p = 0.0002) and low PTENP1 (p = 0.0044) expressions were significantly associated with 5-FU resistance and tumor relapse in stage I/II CC. The invasiveness and colony-forming characteristics of 5-FU-resistant cell lineages were higher as compared to the parent HT-29. Moreover, the expression of MALAT1 (p = 0.0009) was increased while the expression of PTENP1 (p = 0.0158) decreased in 5FU-resistant-HT-29 cells. Si-MALAT1 treatment increased cell sensitivity to 5FU, whereas it decreased invasive behaviors of 5 FU-resistant-HT-29 cells. Conclusion MALAT1 may be a biomarker in predicting recurrence in early-stage CC. Our findings suggest that a cell-based therapy to target MALAT1 could be established for these patients to prevent metastasis and 5-FU resistance. | |
dc.identifier.doi | 10.1007/s11033-022-07680-y | |
dc.identifier.endpage | 11253 | |
dc.identifier.issn | 0301-4851 | |
dc.identifier.issue | 12 | |
dc.identifier.startpage | 11243 | |
dc.identifier.uri | https://doi.org/10.1007/s11033-022-07680-y | |
dc.identifier.uri | https://hdl.handle.net/11452/44688 | |
dc.identifier.volume | 49 | |
dc.identifier.wos | 000824839900003 | |
dc.indexed.wos | WOS.SCI | |
dc.language.iso | en | |
dc.publisher | Springer | |
dc.relation.bap | KUAP(T)-2014/35 | |
dc.relation.bap | KUAP(T)-2015/2 | |
dc.relation.bap | DDP(T)-2018/3 | |
dc.relation.journal | Molecular Biology Reports | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.relation.tubitak | 318S256 | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | Rna | |
dc.subject | Chemoresistance | |
dc.subject | Chemotherapy | |
dc.subject | 5fu-resistance | |
dc.subject | Stage i | |
dc.subject | Ii | |
dc.subject | Colon cancer | |
dc.subject | Lncrnas | |
dc.subject | Malat1 | |
dc.subject | Ptenp1 | |
dc.subject | Science & technology | |
dc.subject | Life sciences & biomedicine | |
dc.subject | Biochemistry & molecular biology | |
dc.title | Early-stage colon cancer with high malat1 expression is associated with the 5-fluorouracil resistance and future metastasis | |
dc.type | Article | |
dspace.entity.type | Publication | |
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