Publication:
Early-stage colon cancer with high malat1 expression is associated with the 5-fluorouracil resistance and future metastasis

dc.contributor.authorÖztürk, Ersin
dc.contributor.buuauthorAksoy, Seçil Ak
dc.contributor.buuauthorTunca, Berrin
dc.contributor.buuauthorTUNCA, BERRİN
dc.contributor.buuauthorErçelik, Melis
dc.contributor.buuauthorTezcan, Gulcin
dc.contributor.buuauthorTEZCAN, GÜLÇİN
dc.contributor.buuauthorÇeçener, Gülşah
dc.contributor.buuauthorÇEÇENER, GÜLŞAH
dc.contributor.buuauthorUĞRAŞ, NESRİN
dc.contributor.buuauthorYILMAZLAR, AHMET TUNCAY
dc.contributor.buuauthorYerci, Omer
dc.contributor.buuauthorYERCİ, ÖMER
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/Patoloji Anabilim Dalı.
dc.contributor.orcid0000-0002-1619-6680
dc.contributor.orcid0000-0002-5956-8755
dc.contributor.orcid0000-0001-8593-5101
dc.contributor.orcid0000-0002-3820-424X
dc.contributor.researcheridADM-8457-2022
dc.contributor.researcheridAAH-3843-2020
dc.date.accessioned2024-09-13T06:03:09Z
dc.date.available2024-09-13T06:03:09Z
dc.date.issued2022-07-06
dc.description.abstractBackground This study aimed to investigate the role of long noncoding RNA (LncRNA) expression profiles to predict relapse and 5-FU response in patients with stage I/II colon cancer (CC). Methods and Results The expression level of 15 LncRNA was analyzed in stage I/II colon tumors of 126 CC patients. To confirm the findings in-vitro, 5FU-resistant HT29 cells were generated by subjecting HT-29 cells to the increasing concentrations of 5FU for 6 months. The 5FU resistance was observed in WST-1 and Annexin V analyses. The colony formation and wound healing assays were assessed to determine the metastatic properties of the cells. Expression levels of LncRNAs and mRNA of EMT-related genes were determined by RT-PCR. The role of LncRNA on metastasis and 5FU sensitivity were confirmed in pcDNA3.0-PTENP1 and si-MALAT1 expressed 5FU-resistant HT29 cell lineages. Results High MALAT1 (p = 0.0002) and low PTENP1 (p = 0.0044) expressions were significantly associated with 5-FU resistance and tumor relapse in stage I/II CC. The invasiveness and colony-forming characteristics of 5-FU-resistant cell lineages were higher as compared to the parent HT-29. Moreover, the expression of MALAT1 (p = 0.0009) was increased while the expression of PTENP1 (p = 0.0158) decreased in 5FU-resistant-HT-29 cells. Si-MALAT1 treatment increased cell sensitivity to 5FU, whereas it decreased invasive behaviors of 5 FU-resistant-HT-29 cells. Conclusion MALAT1 may be a biomarker in predicting recurrence in early-stage CC. Our findings suggest that a cell-based therapy to target MALAT1 could be established for these patients to prevent metastasis and 5-FU resistance.
dc.identifier.doi10.1007/s11033-022-07680-y
dc.identifier.endpage11253
dc.identifier.issn0301-4851
dc.identifier.issue12
dc.identifier.startpage11243
dc.identifier.urihttps://doi.org/10.1007/s11033-022-07680-y
dc.identifier.urihttps://hdl.handle.net/11452/44688
dc.identifier.volume49
dc.identifier.wos000824839900003
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherSpringer
dc.relation.bapKUAP(T)-2014/35
dc.relation.bapKUAP(T)-2015/2
dc.relation.bapDDP(T)-2018/3
dc.relation.journalMolecular Biology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.relation.tubitak318S256
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectRna
dc.subjectChemoresistance
dc.subjectChemotherapy
dc.subject5fu-resistance
dc.subjectStage i
dc.subjectIi
dc.subjectColon cancer
dc.subjectLncrnas
dc.subjectMalat1
dc.subjectPtenp1
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectBiochemistry & molecular biology
dc.titleEarly-stage colon cancer with high malat1 expression is associated with the 5-fluorouracil resistance and future metastasis
dc.typeArticle
dspace.entity.typePublication
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relation.isAuthorOfPublicatione171a866-0a2e-4df4-9f4b-d9058971c979
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relation.isAuthorOfPublication.latestForDiscovery121a3732-be5d-4aff-9195-357c8347daca

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