Publication:
Overexpression of dual-specificity phosphatases 4 and 13 attenuates transforming growth factor β1-induced migration and drug resistance in A549 cells in vitro

dc.contributor.authorGüler, Sabire
dc.contributor.authorAltunok, Tuğba H.
dc.contributor.authorSarıoğlu, Aybike
dc.contributor.authorZik, Berrin
dc.contributor.authorAşmaz, Deniz
dc.contributor.authorKayapunar, Nuray
dc.contributor.authorSönmez, Öner
dc.contributor.authorTepedelen, Burcu Erbaykent
dc.contributor.authorYalçın, Abdullah
dc.contributor.buuauthorGÜLER, SABİRE
dc.contributor.buuauthorAltunok, Tuğba H.
dc.contributor.buuauthorSarıoğlu, Aybike
dc.contributor.buuauthorZIK, BERRİN
dc.contributor.buuauthorAşmaz, Deniz
dc.contributor.buuauthorKayapunar, Nuray
dc.contributor.buuauthorSÖNMEZ, ÖNER
dc.contributor.buuauthorTepedelen, Burcu Erbaykent
dc.contributor.buuauthorYALÇIN, ABDULLAH
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi/Histoloji ve Embriyoloji Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi/Biyokimya Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Moleküler Biyoloji ve Genetik Anabilim Dalı.
dc.contributor.orcid0000-0002-7367-6859
dc.contributor.orcid0000-0003-1263-3799
dc.contributor.orcid0000-0002-1062-332X
dc.contributor.orcid0000-0001-8519-8375
dc.contributor.orcid0000-0002-8287-6617
dc.contributor.orcid0000-0001-6468-8535
dc.contributor.researcheridAAH-8807-2021
dc.contributor.researcheridKMY-2643-2024
dc.contributor.researcheridS-2474-2018
dc.contributor.researcheridAAH-9810-2021
dc.contributor.researcheridHPG-0648-2023
dc.contributor.researcheridGXM-5514-2022
dc.contributor.researcheridDTN-7054-2022
dc.contributor.researcheridAAH-6436-2021
dc.contributor.researcheridABI-4164-2020
dc.date.accessioned2024-10-02T07:39:08Z
dc.date.available2024-10-02T07:39:08Z
dc.date.issued2022-03-23
dc.description.abstractTransforming growth factor-beta (TGF beta) proteins induce an epithelial-mesenchymal transition (EMT) programme that is associated with increased invasive and drug-resistant phenotype of carcinoma cells. In addition to the canonical pathway involving SMAD proteins, the mitogen-activated kinase (MAPK) pathway via extracellular signal-regulated kinases 1/2 (ERK1/2) is also involved in promoting and maintaining a mesenchymal phenotype by tumor cells following TGF beta signal activation. As dual-specificity phosphatases (DUSPs) regulate ERK1/2 activity by dephosphorylation, we aimed to examine DUSPs' expression upon TGF beta stimulation and whether DUSPs play a role in the EMT and related phenotypes promoted by TGF beta 1 in A549 cells. We found that TGF beta 1 stimulation led to marked changes in several DUSP proteins, including significant decreases in DUSP4 and DUSP13 expressions. We then showed that the ectopic co-expression of DUSP4/13 suppresses TGF beta 1-induced ERK1/2 phosphorylation and protein levels of the EMT transcription factors Snail and Slug proteins. We then demonstrated that DUSP4/13 co-expression partially inhibited TGF beta 1-promoted migration, invasion, and chemoresistance in A549 cells. Collectively, this report provides data for the involvement of DUSP4/13 in malignant phenotypes regulated by TGF beta 1 in A549 cells. (C) 2022 Elsevier Inc. All rights reserved.
dc.identifier.doi10.1016/j.bbrc.2022.03.090
dc.identifier.endpage41
dc.identifier.issn0006-291X
dc.identifier.startpage35
dc.identifier.urihttps://doi.org/10.1016/j.bbrc.2022.03.090
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0006291X22004235?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/11452/45644
dc.identifier.volume606
dc.identifier.wos000791590000007
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Science
dc.relation.journalBiochemical and Biophysical Research Communications
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.relation.tubitak118Z092
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectEpithelial-mesenchymal transition
dc.subjectTranscription
dc.subjectBeta
dc.subjectEmt
dc.subjectTransforming growth factor beta 1
dc.subjectEpithelial-mesenchymal transition
dc.subjectDual-specificity phosphatase 4
dc.subjectDual-specificity phosphatase 13
dc.subjectExtracellular signal-regulated kinase
dc.subjectBiochemistry & molecular biology
dc.subjectBiophysics
dc.titleOverexpression of dual-specificity phosphatases 4 and 13 attenuates transforming growth factor β1-induced migration and drug resistance in A549 cells in vitro
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication65c482b5-8e65-438b-934f-90fef538a967
relation.isAuthorOfPublicationa30427a9-dc3b-4196-927b-d5b5b4e35e1f
relation.isAuthorOfPublication0ebc6304-72ad-40ef-913e-a95ae0112df1
relation.isAuthorOfPublication24332407-6513-4c39-92c2-21a376f853b1
relation.isAuthorOfPublication.latestForDiscovery65c482b5-8e65-438b-934f-90fef538a967

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