Publication: Overexpression of dual-specificity phosphatases 4 and 13 attenuates transforming growth factor β1-induced migration and drug resistance in A549 cells in vitro
dc.contributor.author | Güler, Sabire | |
dc.contributor.author | Altunok, Tuğba H. | |
dc.contributor.author | Sarıoğlu, Aybike | |
dc.contributor.author | Zik, Berrin | |
dc.contributor.author | Aşmaz, Deniz | |
dc.contributor.author | Kayapunar, Nuray | |
dc.contributor.author | Sönmez, Öner | |
dc.contributor.author | Tepedelen, Burcu Erbaykent | |
dc.contributor.author | Yalçın, Abdullah | |
dc.contributor.buuauthor | GÜLER, SABİRE | |
dc.contributor.buuauthor | Altunok, Tuğba H. | |
dc.contributor.buuauthor | Sarıoğlu, Aybike | |
dc.contributor.buuauthor | ZIK, BERRİN | |
dc.contributor.buuauthor | Aşmaz, Deniz | |
dc.contributor.buuauthor | Kayapunar, Nuray | |
dc.contributor.buuauthor | SÖNMEZ, ÖNER | |
dc.contributor.buuauthor | Tepedelen, Burcu Erbaykent | |
dc.contributor.buuauthor | YALÇIN, ABDULLAH | |
dc.contributor.department | Veteriner Fakültesi | |
dc.contributor.department | Biyokimya Ana Bilim Dalı | |
dc.contributor.orcid | 0000-0002-7367-6859 | |
dc.contributor.orcid | 0000-0003-1263-3799 | |
dc.contributor.orcid | 0000-0002-1062-332X | |
dc.contributor.orcid | 0000-0001-8519-8375 | |
dc.contributor.orcid | 0000-0002-8287-6617 | |
dc.contributor.orcid | 0000-0001-6468-8535 | |
dc.contributor.researcherid | AAH-8807-2021 | |
dc.contributor.researcherid | KMY-2643-2024 | |
dc.contributor.researcherid | S-2474-2018 | |
dc.contributor.researcherid | AAH-9810-2021 | |
dc.contributor.researcherid | HPG-0648-2023 | |
dc.contributor.researcherid | GXM-5514-2022 | |
dc.contributor.researcherid | DTN-7054-2022 | |
dc.contributor.researcherid | AAH-6436-2021 | |
dc.contributor.researcherid | ABI-4164-2020 | |
dc.date.accessioned | 2024-10-02T07:39:08Z | |
dc.date.available | 2024-10-02T07:39:08Z | |
dc.date.issued | 2022-03-23 | |
dc.description.abstract | Transforming growth factor-beta (TGF beta) proteins induce an epithelial-mesenchymal transition (EMT) programme that is associated with increased invasive and drug-resistant phenotype of carcinoma cells. In addition to the canonical pathway involving SMAD proteins, the mitogen-activated kinase (MAPK) pathway via extracellular signal-regulated kinases 1/2 (ERK1/2) is also involved in promoting and maintaining a mesenchymal phenotype by tumor cells following TGF beta signal activation. As dual-specificity phosphatases (DUSPs) regulate ERK1/2 activity by dephosphorylation, we aimed to examine DUSPs' expression upon TGF beta stimulation and whether DUSPs play a role in the EMT and related phenotypes promoted by TGF beta 1 in A549 cells. We found that TGF beta 1 stimulation led to marked changes in several DUSP proteins, including significant decreases in DUSP4 and DUSP13 expressions. We then showed that the ectopic co-expression of DUSP4/13 suppresses TGF beta 1-induced ERK1/2 phosphorylation and protein levels of the EMT transcription factors Snail and Slug proteins. We then demonstrated that DUSP4/13 co-expression partially inhibited TGF beta 1-promoted migration, invasion, and chemoresistance in A549 cells. Collectively, this report provides data for the involvement of DUSP4/13 in malignant phenotypes regulated by TGF beta 1 in A549 cells. (C) 2022 Elsevier Inc. All rights reserved. | |
dc.identifier.doi | 10.1016/j.bbrc.2022.03.090 | |
dc.identifier.endpage | 41 | |
dc.identifier.issn | 0006-291X | |
dc.identifier.startpage | 35 | |
dc.identifier.uri | https://doi.org/10.1016/j.bbrc.2022.03.090 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0006291X22004235?via%3Dihub | |
dc.identifier.uri | https://hdl.handle.net/11452/45644 | |
dc.identifier.volume | 606 | |
dc.identifier.wos | 000791590000007 | |
dc.indexed.wos | WOS.SCI | |
dc.language.iso | en | |
dc.publisher | Academic Press Inc Elsevier Science | |
dc.relation.journal | Biochemical and Biophysical Research Communications | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.relation.tubitak | 118Z092 | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | Epithelial-mesenchymal transition | |
dc.subject | Transcription | |
dc.subject | Beta | |
dc.subject | Emt | |
dc.subject | Transforming growth factor beta 1 | |
dc.subject | Epithelial-mesenchymal transition | |
dc.subject | Dual-specificity phosphatase 4 | |
dc.subject | Dual-specificity phosphatase 13 | |
dc.subject | Extracellular signal-regulated kinase | |
dc.subject | Biochemistry & molecular biology | |
dc.subject | Biophysics | |
dc.title | Overexpression of dual-specificity phosphatases 4 and 13 attenuates transforming growth factor β1-induced migration and drug resistance in A549 cells in vitro | |
dc.type | Article | |
dspace.entity.type | Publication | |
local.contributor.department | Veteriner Fakültesi/Histoloji ve Embriyoloji Ana Bilim Dalı | |
local.contributor.department | Veteriner Fakültesi/Biyokimya Ana Bilim Dalı | |
local.contributor.department | Fen Edebiyat Fakültesi/Moleküler Biyoloji ve Genetik Ana Bilim Dalı | |
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relation.isAuthorOfPublication.latestForDiscovery | 65c482b5-8e65-438b-934f-90fef538a967 |