Publication:
The cytotoxic and antitumoral effects of Remdesivir, an antiviral RdRp inhibitor, on different cancer cells in vitro

dc.contributor.authorEryılmaz, Işıl Ezgi
dc.contributor.authorBergel, Ceyda Çolakoğlu
dc.contributor.authorEgeli, Ünal
dc.contributor.authorÇeçener, Gülşah
dc.contributor.buuauthorERYILMAZ, IŞIL EZGİ
dc.contributor.buuauthorBergel, Ceyda Çolakoğlu
dc.contributor.buuauthorEGELİ, ÜNAL
dc.contributor.buuauthorÇEÇENER, GÜLŞAH
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji Anabilim Dalı
dc.contributor.orcid0000-0002-7471-5071
dc.contributor.orcid0000-0001-7904-883X
dc.contributor.orcid0000-0002-3820-424X
dc.contributor.researcheridGWV-3548-2022
dc.contributor.researcheridAEZ-2955-2022
dc.contributor.researcheridAAH-1420-2021
dc.contributor.researcheridAAP-9988-2020
dc.date.accessioned2024-10-07T10:45:55Z
dc.date.available2024-10-07T10:45:55Z
dc.date.issued2023-07-22
dc.description.abstractBackgroundPrevious studies have shown similarities in the metabolism of cancer cells and parasites, suggesting that antiparasitic drugs may be used as anticancer agents. Remdesivir (Rem), an RdRp inhibitor, has been recently used in SARS-CoV-2 pandemic. Although the apoptotic effect of Rem has been demonstrated on the SKOV3 ovarian cancer cell line, its cytotoxic effect has not been analyzed in different cancer cells.ObjectiveWe aimed to evaluate its cell death-inducing effects on PC3 prostate cancer, HepG2 hepatocellular carcinoma, and A2058 malignant melanoma cells for the first time, using WST-1, Annexin V, cell cycle analysis, AO/EB staining, live-cell imaging, TEM, and gene expression analysis.ResultsRem treatment at 10, 25, and 50 & mu;M significantly decreased cell viability in all cancer cells (p < 0.01). While Rem triggered apoptosis in PC3, vacuole-dependent cell death was detected in HepG2 cells by visualizing the cells with TEM and time-dependent live-cell imaging. Moreover, both forms of cell death were triggered together in A2058. The formation of AVOs were observed more after 12 h and 24 h in HepG2 and A2058 cells, respectively. Additionally, Rem significantly induced cell cycle arrest in the G2/M (p < 0.01). Finally, the mRNA levels of autophagic markers, Atg12, Atg5, p62, Beclin, and LC3, were significantly increased in A2058 and HepG2 (p < 0.01) compared to control groups.ConclusionsOur results suggest that Rem has promising cytotoxic effects on various cancer cells. However, it triggers different types of cell death in different cancer types, indicating that further studies should focus on clarifying the molecular mechanism of the specific action of Rem.
dc.identifier.doi10.1007/s13273-023-00379-6
dc.identifier.eissn2092-8467
dc.identifier.endpage660
dc.identifier.govdochttps://link.springer.com/article/10.1007/s13273-023-00379-6
dc.identifier.issn1738-642X
dc.identifier.issue3
dc.identifier.startpage649
dc.identifier.urihttps://doi.org/10.1007/s13273-023-00379-6
dc.identifier.urihttps://link.springer.com/article/10.1007/s13273-023-00379-6
dc.identifier.urihttps://hdl.handle.net/11452/45970
dc.identifier.volume20
dc.identifier.wos001034561800001
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherKorean Society Toxicogenomics & Toxicoproteomics-kstt
dc.relation.bapTHIZ-2021599
dc.relation.journalMolecular & Cellular Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDaphnia-magna
dc.subjectWater
dc.subjectMicroplastics
dc.subjectNanoplastics
dc.subjectParticles
dc.subjectIngestion
dc.subjectExposure
dc.subjectImmobilization
dc.subjectReproduction
dc.subjectRemdesivir
dc.subjectCancer
dc.subjectCytotoxic effect
dc.subjectApoptosis
dc.subjectAutophagy
dc.subjectCell death
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectBiochemistry & molecular biology
dc.subjectToxicology
dc.titleThe cytotoxic and antitumoral effects of Remdesivir, an antiviral RdRp inhibitor, on different cancer cells in vitro
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication134440c4-386b-47a8-a04b-f11708a8cab2
relation.isAuthorOfPublication051cf631-d214-4c8f-b1f5-fa1d27d5269c
relation.isAuthorOfPublicationae26ce61-4a33-4336-9fe3-b40d1138c397
relation.isAuthorOfPublication.latestForDiscovery134440c4-386b-47a8-a04b-f11708a8cab2

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