Publication:
Investigation of miR-146a expression profiles in fecal samples of patients with multiple sclerosis for early diagnosis and treatment

dc.contributor.authorÜnlü, Havva Tezcan
dc.contributor.authorSarıdaş, Furkan
dc.contributor.authorTaşkapılıoğlu, Özlem
dc.contributor.authorÇeçener, Gülşah
dc.contributor.authorEgeli, Ünal
dc.contributor.authorTuran, Ömer Faruk
dc.contributor.authorTunca, Berrin
dc.contributor.authorZarifoğlu, Mehmet
dc.contributor.buuauthorÜnlü, Havva Tezcan
dc.contributor.buuauthorSARIDAŞ, FURKAN
dc.contributor.buuauthorTaşkapılıoğlu, Özlem
dc.contributor.buuauthorTURAN, ÖMER FARUK
dc.contributor.buuauthorÇEÇENER, GÜLŞAH
dc.contributor.buuauthorEGELİ, ÜNAL
dc.contributor.buuauthorTUNCA, BERRİN
dc.contributor.buuauthorZARİFOĞLU, MEHMET
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji Anabilim Dalı.
dc.contributor.departmentBursa Uludağ Üniversitesi/Tıp Fakültesi/Nöroloji Anabilim Dalı.
dc.contributor.orcid0000-0002-0910-4258
dc.contributor.orcid0000-0001-5945-2317
dc.contributor.orcid0000-0002-3820-424X
dc.contributor.orcid0000-0001-7904-883X
dc.contributor.orcid0000-0002-1619-6680
dc.contributor.researcheridGYU-0252-2022
dc.contributor.researcheridHSB-2700-2023
dc.contributor.researcheridEBA-4926-2022
dc.contributor.researcheridAAP-9988-2020
dc.contributor.researcheridAAH-1420-2021
dc.contributor.researcheridJDI-6091-2023
dc.contributor.researcheridABI-6078-2020
dc.contributor.researcheridEHN-5825-2022
dc.date.accessioned2024-10-03T05:21:51Z
dc.date.available2024-10-03T05:21:51Z
dc.date.issued2023-04-01
dc.description.abstractIntroduction: Recent research into multiple sclerosis (MS) has focused on the role of microRNAs (miRNAs) in the development of the disease. This study was designed to analyze miR-146a expression in whole blood and fecal samples of patients with MS. The study aimed to analyze clinical data using the miR-146a expression values obtained. Subjects and Methods: This study included patients with relapsing-remitting MS (RRMS) (n = 53), clinically isolated syndrome (CIS) (n = 15), and healthy controls (n = 26). Total RNA was isolated from the participants' whole blood and fecal samples. RNA extraction was performed using QIAamp RNA Blood Mini Kits for blood samples and RNeasy PowerMicrobiome Kits for feces. miR-146a expressions were studied using real-time polymerase chain reaction. Finally, relative expression was correlated with clinicopathologic factors. Results: MiR-146a expression was significantly decreased in the whole blood (P < 0.001) and fecal samples (P = 0.036) of patients with RRMS. There was no significant difference in the miR-146a expression rate between patients with CIS and controls. Moreover, the miR-146a expression level in patients with RRMS was decreased compared with those with CIS (P < 0.001). A significant association was determined between miR-146a expression and sex in blood samples. When sex stratification was applied to expression values obtained from fecal samples, miR-146a expression was downregulated only in females (P = 0.008). Discussion: miRNAs play an essential role in maintaining the stable course of MS, and this process has some sex-specific differences. Expression of fecal miR-146a may be used as a biomarker to diagnose and predict prognosis in patients with RRMS.
dc.description.sponsorshipCouncil of Higher Education (CoHE)
dc.identifier.doi10.4103/nsn.nsn_218_22
dc.identifier.endpage87
dc.identifier.issn2636-865X
dc.identifier.issue2
dc.identifier.startpage81
dc.identifier.urihttps://doi.org/10.4103/nsn.nsn_218_22
dc.identifier.urihttps://journals.lww.com/nsan/fulltext/2023/40020/investigation_of_mir_146a_expression_profiles_in.4.aspx
dc.identifier.urihttps://hdl.handle.net/11452/45721
dc.identifier.volume40
dc.identifier.wos001026535300004
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherWolters Kluwer Medknow Publications
dc.relation.bapOUAP (T)-2015/23
dc.relation.journalNeurological Sciences and Neurophysiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMicrornas
dc.subjectImmune
dc.subjectRisk
dc.subjectFecal microrna
dc.subjectGut microbiome
dc.subjectMir-146a
dc.subjectMultiple sclerosis
dc.subjectRelapsing-remitting multiple sclerosis
dc.subjectNeurosciences & neurology
dc.titleInvestigation of miR-146a expression profiles in fecal samples of patients with multiple sclerosis for early diagnosis and treatment
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication80b8fcdf-d916-4b3e-a7cb-00aebac954a7
relation.isAuthorOfPublicationae26ce61-4a33-4336-9fe3-b40d1138c397
relation.isAuthorOfPublication051cf631-d214-4c8f-b1f5-fa1d27d5269c
relation.isAuthorOfPublication121a3732-be5d-4aff-9195-357c8347daca
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relation.isAuthorOfPublication.latestForDiscovery80b8fcdf-d916-4b3e-a7cb-00aebac954a7

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