Publication: Presence of Salmonella in retail grade a eggs determined by the International Organization for Standardization 6579 method and a LightCycler polymerase chain reaction system
dc.contributor.author | Temelli, Seran | |
dc.contributor.author | Kahya, Serpil | |
dc.contributor.author | Ata, Zafer | |
dc.contributor.author | Çarlı, Kamil Tayfun | |
dc.contributor.author | Eyigör, Ayşegül | |
dc.contributor.buuauthor | TEMELLİ, SERAN | |
dc.contributor.buuauthor | KAHYA DEMİRBİLEK, SERPİL | |
dc.contributor.buuauthor | Ata, Zafer | |
dc.contributor.buuauthor | CARLI, KAMİL TAYFUN | |
dc.contributor.buuauthor | Eyigör, Ayşegül | |
dc.contributor.department | Uludağ Üniversitesi/Veteriner Fakültesi. | |
dc.contributor.department | Uludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojisi Anabilim Dalı. | |
dc.contributor.department | Uludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı. | |
dc.contributor.researcherid | AAI-1092-2021 | |
dc.contributor.researcherid | W-7994-2019 | |
dc.contributor.researcherid | AAH-2842-2021 | |
dc.contributor.researcherid | AAI-1101-2021 | |
dc.contributor.researcherid | AAH-2842-2021 | |
dc.date.accessioned | 2024-08-06T05:43:43Z | |
dc.date.available | 2024-08-06T05:43:43Z | |
dc.date.issued | 2015-01-01 | |
dc.description.abstract | This study aims to determine the presence of Salmonella in naturally contaminated grade A eggs by the standard culture method International Organization for Standardization Method 6579 (ISO) and a specific real-time PCR system (LightCycler PCR-LCPCR) to complement ISO. A total of 1635 eggs pooled into 101 samples were randomly collected within one year period from 20 different retail markets in Bursa, Turkey, carrying eggs of 16 large egg producers/suppliers of 5 cities with intensive layer production. Preparation of the egg and shell for analyses, Salmonella isolations and identifications, and detections were performed according to ISO 6887-4:2003, ISO 6579 and LCPCR, respectively. Overall Salmonella detection rate by ISO and LCPCR were 15.8 % (16/101) and 46.5 % (47/101), respectively. Out of 101 inner parts, Salmonella was detected in 11 (10.9 %) samples by ISO, and in 31 (30.7 %) samples by LCPCR. Six of 101 shell samples (5.9 %) were found to harbor Salmonella by ISO, while 18 (17.8 %) shells were positive by LCPCR. All isolates were determined as Salmonella enterica subsp. enterica serovar Enteritidis. These findings indicate considerably high Salmonella contamination in retail grade A eggs. This should be under routine monitoring by rapid methods such as PCR, complemented by standard culture to evaluate and assess the significance of risk for public health. | |
dc.identifier.doi | 10.1501/Vetfak_0000002669 | |
dc.identifier.endpage | 132 | |
dc.identifier.issn | 1300-0861 | |
dc.identifier.issue | 2 | |
dc.identifier.startpage | 125 | |
dc.identifier.uri | http://vetjournal.ankara.edu.tr/tr/pub/issue/43546/532398 | |
dc.identifier.uri | https://doi.org/10.1501/Vetfak_0000002669 | |
dc.identifier.uri | https://hdl.handle.net/11452/43730 | |
dc.identifier.volume | 62 | |
dc.identifier.wos | 000350431800008 | |
dc.indexed.wos | WOS.SCI | |
dc.language.iso | en | |
dc.publisher | Ankara Üniversitesi | |
dc.relation.bap | 2010/10 | |
dc.relation.journal | Ankara Universitesi Veteriner Fakultesi Dergisi | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | Real-time PCR | |
dc.subject | Culture methods | |
dc.subject | Antimicrobial resistance | |
dc.subject | Shell eggs | |
dc.subject | Prevalence | |
dc.subject | Poultry | |
dc.subject | Iso 6579 | |
dc.subject | Real-time polymerase chain reaction | |
dc.subject | Retail EGG | |
dc.subject | Salmonella | |
dc.subject | Veterinary sciences | |
dc.title | Presence of Salmonella in retail grade a eggs determined by the International Organization for Standardization 6579 method and a LightCycler polymerase chain reaction system | |
dc.type | Article | |
dspace.entity.type | Publication | |
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relation.isAuthorOfPublication | c131131a-7bfa-4fdb-a81a-f4b59c53a2d6 | |
relation.isAuthorOfPublication | cd431f1b-601a-401d-b8e4-f4b7c734ec85 | |
relation.isAuthorOfPublication.latestForDiscovery | 0750c1c3-d224-463a-9700-2b3f81d9731c |