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Presence of Salmonella in retail grade a eggs determined by the International Organization for Standardization 6579 method and a LightCycler polymerase chain reaction system

dc.contributor.authorTemelli, Seran
dc.contributor.authorKahya, Serpil
dc.contributor.authorAta, Zafer
dc.contributor.authorÇarlı, Kamil Tayfun
dc.contributor.authorEyigör, Ayşegül
dc.contributor.buuauthorTEMELLİ, SERAN
dc.contributor.buuauthorKAHYA DEMİRBİLEK, SERPİL
dc.contributor.buuauthorAta, Zafer
dc.contributor.buuauthorCARLI, KAMİL TAYFUN
dc.contributor.buuauthorEyigör, Ayşegül
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi.
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/Gıda Hijyeni ve Teknolojisi Anabilim Dalı.
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/Mikrobiyoloji Anabilim Dalı.
dc.contributor.researcheridAAI-1092-2021
dc.contributor.researcheridW-7994-2019
dc.contributor.researcheridAAH-2842-2021
dc.contributor.researcheridAAI-1101-2021
dc.contributor.researcheridAAH-2842-2021
dc.date.accessioned2024-08-06T05:43:43Z
dc.date.available2024-08-06T05:43:43Z
dc.date.issued2015-01-01
dc.description.abstractThis study aims to determine the presence of Salmonella in naturally contaminated grade A eggs by the standard culture method International Organization for Standardization Method 6579 (ISO) and a specific real-time PCR system (LightCycler PCR-LCPCR) to complement ISO. A total of 1635 eggs pooled into 101 samples were randomly collected within one year period from 20 different retail markets in Bursa, Turkey, carrying eggs of 16 large egg producers/suppliers of 5 cities with intensive layer production. Preparation of the egg and shell for analyses, Salmonella isolations and identifications, and detections were performed according to ISO 6887-4:2003, ISO 6579 and LCPCR, respectively. Overall Salmonella detection rate by ISO and LCPCR were 15.8 % (16/101) and 46.5 % (47/101), respectively. Out of 101 inner parts, Salmonella was detected in 11 (10.9 %) samples by ISO, and in 31 (30.7 %) samples by LCPCR. Six of 101 shell samples (5.9 %) were found to harbor Salmonella by ISO, while 18 (17.8 %) shells were positive by LCPCR. All isolates were determined as Salmonella enterica subsp. enterica serovar Enteritidis. These findings indicate considerably high Salmonella contamination in retail grade A eggs. This should be under routine monitoring by rapid methods such as PCR, complemented by standard culture to evaluate and assess the significance of risk for public health.
dc.identifier.doi10.1501/Vetfak_0000002669
dc.identifier.endpage132
dc.identifier.issn1300-0861
dc.identifier.issue2
dc.identifier.startpage125
dc.identifier.urihttp://vetjournal.ankara.edu.tr/tr/pub/issue/43546/532398
dc.identifier.urihttps://doi.org/10.1501/Vetfak_0000002669
dc.identifier.urihttps://hdl.handle.net/11452/43730
dc.identifier.volume62
dc.identifier.wos000350431800008
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherAnkara Üniversitesi
dc.relation.bap2010/10
dc.relation.journalAnkara Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectReal-time PCR
dc.subjectCulture methods
dc.subjectAntimicrobial resistance
dc.subjectShell eggs
dc.subjectPrevalence
dc.subjectPoultry
dc.subjectIso 6579
dc.subjectReal-time polymerase chain reaction
dc.subjectRetail EGG
dc.subjectSalmonella
dc.subjectVeterinary sciences
dc.titlePresence of Salmonella in retail grade a eggs determined by the International Organization for Standardization 6579 method and a LightCycler polymerase chain reaction system
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication0750c1c3-d224-463a-9700-2b3f81d9731c
relation.isAuthorOfPublicationc131131a-7bfa-4fdb-a81a-f4b59c53a2d6
relation.isAuthorOfPublicationcd431f1b-601a-401d-b8e4-f4b7c734ec85
relation.isAuthorOfPublication.latestForDiscovery0750c1c3-d224-463a-9700-2b3f81d9731c

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