Publication:
Transforming growth factor β1 upregulates 6‐phosphofructo‐ 2‐kinase/fructose 2,6‐bisphosphatase‐4 expression in A549 and MCF‐10A cells

dc.contributor.authorAltunok, Tuğba H.
dc.contributor.authorMuchut, Robertino J.
dc.contributor.authorIglesias, Alberto A.
dc.contributor.authorYalçın, Abdullah
dc.contributor.buuauthorAltunok, Tuğba H.
dc.contributor.buuauthorYALÇIN, ABDULLAH
dc.contributor.departmentBursa Uludağ Üniversitesi/Veterinerlik Fakültesi/Biyokimya Anabilim Dalı.
dc.contributor.orcid0000-0003-1263-3799
dc.contributor.researcheridKMY-2643-2024
dc.contributor.researcheridJTP-1429-2023
dc.date.accessioned2024-09-10T12:50:27Z
dc.date.available2024-09-10T12:50:27Z
dc.date.issued2023-09-04
dc.description.abstractTransforming growth factor beta 1 (TGF beta 1) induces a cellular process known as epithelial-mesenchymal transition (EMT) associated with metabolic reprogramming, including enhanced glycolysis. Given the involvement of 6-phosphofructo-2-kinase/ fructose 2,6-bisphosphatase (PFKFB) enzymes in glycolysis, we aimed to investigate whether TGF beta 1 regulates expressions of PFKFB genes and if PFKFBs are required for TGF beta 1-driven phenotypes. A549 and MCF-10A cell lines were used as TGF beta 1driven EMT models. Messenger RNA expressions of PFKFB and EMT genes were determined by real-time quantitative polymerase chain reaction. A small interfering RNA approach was used to deplete PFKFB4 expression. A Matrigel invasion assay was conducted to assess the effect of PFKFB4 silencing on the TGF ss 1-enhanced invasion of A549 cells. F2,6BP levels were analyzed using an enzyme-coupled assay. Glucose and lactate concentrations were determined using colorimetric assays. TGF beta 1 robustly induced expression of the fourth isoform of PFKFBs, PFKFB4, in both cell lines. PFKFB4 depletion partially inhibits mesenchymal transdifferentiation caused by TGF beta 1 in A549 cells, as assessed by microscopy. Inductions of Snail in MCF-10A cells and Fibronectin in A549 cells and repressions of E-cadherin in both cell lines by TGF beta 1 are attenuated by PFKFB4 silencing. PFKFB4 silencing reduces F2,6BP and glycolytic activity, although TGF beta 1 alone does not affect these parameters. Finally, PFKFB4 depletion suppresses the TGF beta 1-driven invasion of A549 cells through Matrigel. Presented data suggest that TGF ss 1 induces the expression of PFKFB4 in A549 and MCF-10 cells, and PFKFB4 may be required for TGF beta 1-driven phenotypes such as EMT and invasion in these models.
dc.identifier.doi10.1002/cbf.3856
dc.identifier.endpage1229
dc.identifier.issn0263-6484
dc.identifier.issue8
dc.identifier.startpage1220
dc.identifier.urihttps://doi.org/10.1002/cbf.3856
dc.identifier.urihttps://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/cbf.3856
dc.identifier.urihttps://hdl.handle.net/11452/44511
dc.identifier.volume41
dc.identifier.wos001066730000001
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherWiley
dc.relation.journalCell Biochemistry and Function
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.relation.tubitak113Z776
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectEmt
dc.subjectGlycolysis
dc.subjectMetabolism
dc.subjectPathway
dc.subjectPfkfb3
dc.subjectEpithelial-mesenchymal transition
dc.subjectGlycolysis
dc.subjectPfkfb4
dc.subjectTgf beta 1
dc.subjectBiochemistry & molecular biology
dc.subjectCell biology
dc.titleTransforming growth factor β1 upregulates 6‐phosphofructo‐ 2‐kinase/fructose 2,6‐bisphosphatase‐4 expression in A549 and MCF‐10A cells
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication24332407-6513-4c39-92c2-21a376f853b1
relation.isAuthorOfPublication.latestForDiscovery24332407-6513-4c39-92c2-21a376f853b1

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