Publication:
Transforming growth factor β1 upregulates 6‐phosphofructo‐ 2‐kinase/fructose 2,6‐bisphosphatase‐4 expression in A549 and MCF‐10A cells

dc.contributor.authorAltunok, Tuğba H.
dc.contributor.authorMuchut, Robertino J.
dc.contributor.authorIglesias, Alberto A.
dc.contributor.authorYalçın, Abdullah
dc.contributor.buuauthorAltunok, Tuğba H.
dc.contributor.buuauthorYALÇIN, ABDULLAH
dc.contributor.departmentVeterinerlik Fakültesi
dc.contributor.departmentBiyokimya Ana Bilim Dalı
dc.contributor.orcid0000-0003-1263-3799
dc.contributor.researcheridKMY-2643-2024
dc.contributor.researcheridJTP-1429-2023
dc.date.accessioned2024-09-10T12:50:27Z
dc.date.available2024-09-10T12:50:27Z
dc.date.issued2023-09-04
dc.description.abstractTransforming growth factor beta 1 (TGF beta 1) induces a cellular process known as epithelial-mesenchymal transition (EMT) associated with metabolic reprogramming, including enhanced glycolysis. Given the involvement of 6-phosphofructo-2-kinase/ fructose 2,6-bisphosphatase (PFKFB) enzymes in glycolysis, we aimed to investigate whether TGF beta 1 regulates expressions of PFKFB genes and if PFKFBs are required for TGF beta 1-driven phenotypes. A549 and MCF-10A cell lines were used as TGF beta 1driven EMT models. Messenger RNA expressions of PFKFB and EMT genes were determined by real-time quantitative polymerase chain reaction. A small interfering RNA approach was used to deplete PFKFB4 expression. A Matrigel invasion assay was conducted to assess the effect of PFKFB4 silencing on the TGF ss 1-enhanced invasion of A549 cells. F2,6BP levels were analyzed using an enzyme-coupled assay. Glucose and lactate concentrations were determined using colorimetric assays. TGF beta 1 robustly induced expression of the fourth isoform of PFKFBs, PFKFB4, in both cell lines. PFKFB4 depletion partially inhibits mesenchymal transdifferentiation caused by TGF beta 1 in A549 cells, as assessed by microscopy. Inductions of Snail in MCF-10A cells and Fibronectin in A549 cells and repressions of E-cadherin in both cell lines by TGF beta 1 are attenuated by PFKFB4 silencing. PFKFB4 silencing reduces F2,6BP and glycolytic activity, although TGF beta 1 alone does not affect these parameters. Finally, PFKFB4 depletion suppresses the TGF beta 1-driven invasion of A549 cells through Matrigel. Presented data suggest that TGF ss 1 induces the expression of PFKFB4 in A549 and MCF-10 cells, and PFKFB4 may be required for TGF beta 1-driven phenotypes such as EMT and invasion in these models.
dc.identifier.doi10.1002/cbf.3856
dc.identifier.endpage1229
dc.identifier.issn0263-6484
dc.identifier.issue8
dc.identifier.startpage1220
dc.identifier.urihttps://doi.org/10.1002/cbf.3856
dc.identifier.urihttps://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/cbf.3856
dc.identifier.urihttps://hdl.handle.net/11452/44511
dc.identifier.volume41
dc.identifier.wos001066730000001
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherWiley
dc.relation.journalCell Biochemistry and Function
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.relation.tubitak113Z776
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectEmt
dc.subjectGlycolysis
dc.subjectMetabolism
dc.subjectPathway
dc.subjectPfkfb3
dc.subjectEpithelial-mesenchymal transition
dc.subjectGlycolysis
dc.subjectPfkfb4
dc.subjectTgf beta 1
dc.subjectBiochemistry & molecular biology
dc.subjectCell biology
dc.titleTransforming growth factor β1 upregulates 6‐phosphofructo‐ 2‐kinase/fructose 2,6‐bisphosphatase‐4 expression in A549 and MCF‐10A cells
dc.typeArticle
dspace.entity.typePublication
local.contributor.departmentVeterinerlik Fakültesi/Biyokimya Ana Bilim Dalı
relation.isAuthorOfPublication24332407-6513-4c39-92c2-21a376f853b1
relation.isAuthorOfPublication.latestForDiscovery24332407-6513-4c39-92c2-21a376f853b1

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