Publication: Transforming growth factor β1 upregulates 6‐phosphofructo‐ 2‐kinase/fructose 2,6‐bisphosphatase‐4 expression in A549 and MCF‐10A cells
dc.contributor.author | Altunok, Tuğba H. | |
dc.contributor.author | Muchut, Robertino J. | |
dc.contributor.author | Iglesias, Alberto A. | |
dc.contributor.author | Yalçın, Abdullah | |
dc.contributor.buuauthor | Altunok, Tuğba H. | |
dc.contributor.buuauthor | YALÇIN, ABDULLAH | |
dc.contributor.department | Bursa Uludağ Üniversitesi/Veterinerlik Fakültesi/Biyokimya Anabilim Dalı. | |
dc.contributor.orcid | 0000-0003-1263-3799 | |
dc.contributor.researcherid | KMY-2643-2024 | |
dc.contributor.researcherid | JTP-1429-2023 | |
dc.date.accessioned | 2024-09-10T12:50:27Z | |
dc.date.available | 2024-09-10T12:50:27Z | |
dc.date.issued | 2023-09-04 | |
dc.description.abstract | Transforming growth factor beta 1 (TGF beta 1) induces a cellular process known as epithelial-mesenchymal transition (EMT) associated with metabolic reprogramming, including enhanced glycolysis. Given the involvement of 6-phosphofructo-2-kinase/ fructose 2,6-bisphosphatase (PFKFB) enzymes in glycolysis, we aimed to investigate whether TGF beta 1 regulates expressions of PFKFB genes and if PFKFBs are required for TGF beta 1-driven phenotypes. A549 and MCF-10A cell lines were used as TGF beta 1driven EMT models. Messenger RNA expressions of PFKFB and EMT genes were determined by real-time quantitative polymerase chain reaction. A small interfering RNA approach was used to deplete PFKFB4 expression. A Matrigel invasion assay was conducted to assess the effect of PFKFB4 silencing on the TGF ss 1-enhanced invasion of A549 cells. F2,6BP levels were analyzed using an enzyme-coupled assay. Glucose and lactate concentrations were determined using colorimetric assays. TGF beta 1 robustly induced expression of the fourth isoform of PFKFBs, PFKFB4, in both cell lines. PFKFB4 depletion partially inhibits mesenchymal transdifferentiation caused by TGF beta 1 in A549 cells, as assessed by microscopy. Inductions of Snail in MCF-10A cells and Fibronectin in A549 cells and repressions of E-cadherin in both cell lines by TGF beta 1 are attenuated by PFKFB4 silencing. PFKFB4 silencing reduces F2,6BP and glycolytic activity, although TGF beta 1 alone does not affect these parameters. Finally, PFKFB4 depletion suppresses the TGF beta 1-driven invasion of A549 cells through Matrigel. Presented data suggest that TGF ss 1 induces the expression of PFKFB4 in A549 and MCF-10 cells, and PFKFB4 may be required for TGF beta 1-driven phenotypes such as EMT and invasion in these models. | |
dc.identifier.doi | 10.1002/cbf.3856 | |
dc.identifier.endpage | 1229 | |
dc.identifier.issn | 0263-6484 | |
dc.identifier.issue | 8 | |
dc.identifier.startpage | 1220 | |
dc.identifier.uri | https://doi.org/10.1002/cbf.3856 | |
dc.identifier.uri | https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/cbf.3856 | |
dc.identifier.uri | https://hdl.handle.net/11452/44511 | |
dc.identifier.volume | 41 | |
dc.identifier.wos | 001066730000001 | |
dc.indexed.wos | WOS.SCI | |
dc.language.iso | en | |
dc.publisher | Wiley | |
dc.relation.journal | Cell Biochemistry and Function | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.relation.tubitak | 113Z776 | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | Emt | |
dc.subject | Glycolysis | |
dc.subject | Metabolism | |
dc.subject | Pathway | |
dc.subject | Pfkfb3 | |
dc.subject | Epithelial-mesenchymal transition | |
dc.subject | Glycolysis | |
dc.subject | Pfkfb4 | |
dc.subject | Tgf beta 1 | |
dc.subject | Biochemistry & molecular biology | |
dc.subject | Cell biology | |
dc.title | Transforming growth factor β1 upregulates 6‐phosphofructo‐ 2‐kinase/fructose 2,6‐bisphosphatase‐4 expression in A549 and MCF‐10A cells | |
dc.type | Article | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 24332407-6513-4c39-92c2-21a376f853b1 | |
relation.isAuthorOfPublication.latestForDiscovery | 24332407-6513-4c39-92c2-21a376f853b1 |