Publication:
Methionine, cysteine, and butylated hydroxytoluene enhance cryosurvival of ram semen on post-thaw and post-incubation time points

dc.contributor.authorToker, Mehmed Berk
dc.contributor.authorDoğan, İbrahim
dc.contributor.buuauthorTOKER, MEHMED BERK
dc.contributor.buuauthorDOĞAN, İBRAHİM
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi/Üreme ve Suni Tohumlama Anabilim Dalı
dc.contributor.orcid0000-0003-1976-1814
dc.contributor.orcid0000-0003-4033-9749
dc.contributor.researcheridA-2794-2014
dc.contributor.researcheridR-8366-2018
dc.date.accessioned2024-10-01T05:36:30Z
dc.date.available2024-10-01T05:36:30Z
dc.date.issued2023-06-01
dc.description.abstractDespite there have been many experiments conducted about antioxidants, the best sole or combination use of antioxidants to include as a standard ingredient to freezing extenders is yet to be found. This study was designed to investigate the different doses of methionine (2.5 and 5 mM), cysteine (1 and 2 mM), and butylated hydroxytoluene (BHT) (1 and 2 mM) for ram semen cryopreservation on post-thaw and post-incubation (6 h) time points over spermatological parameters. Semen samples were collected from Kivircik rams via electro-ejaculator in breeding season. After essential spermatological evaluations, appropriate samples were pooled then split into 7 equal aliquots to create study groups (antioxidant free control, 2.5 mM methionine, 5 mM methionine, 1 mM cysteine, 2 mM cysteine, 1 mM BHT, and 2 mM BHT). Semen samples were put into French straws (0.25 mL), and freezing procedure (two-step) was conducted via a programmable gamete freezer. At both time points, motility, HOST, PSA-FITC, and TUNEL assays were made to discover the impacts of cryopreservation and incubation process over sperm cells. Antioxidant supplemented groups yielded better results compared to the control groups in terms of various spermatological parameters not only at post-thaw time point but after incubation for 6 h of time. The study demonstrated that supplementing sperm freezing extenders with previous antioxidants may create new approaches to cryopreservation procedures, and through increasing success rate of freezing, fertility results may increase to better results in near future.
dc.identifier.doi10.1007/s11250-023-03642-z
dc.identifier.eissn1573-7438
dc.identifier.issn0049-4747
dc.identifier.issue3
dc.identifier.urihttps://doi.org/10.1007/s11250-023-03642-z
dc.identifier.urihttps://link.springer.com/article/10.1007/s11250-023-03642-z
dc.identifier.urihttps://assets-eu.researchsquare.com/files/rs-2120718/v1/e04b7bba-a564-4277-85e5-21b8b53b07f2.pdf
dc.identifier.urihttps://hdl.handle.net/11452/45549
dc.identifier.volume55
dc.identifier.wos000992773700004
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherSpringer
dc.relation.journalTropical Animal Health and Production
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectOxidative stress parameters
dc.subjectLecithin-based extenders
dc.subjectAntioxidants
dc.subjectSperm
dc.subjectQuality
dc.subjectPlasma
dc.subjectAntioxidants
dc.subjectCryopreservation
dc.subjectDna integrity
dc.subjectIncubation resilience
dc.subjectRam semen
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectAgriculture, dairy & animal science
dc.subjectVeterinary sciences
dc.subjectAgriculture
dc.titleMethionine, cysteine, and butylated hydroxytoluene enhance cryosurvival of ram semen on post-thaw and post-incubation time points
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication3224fce1-4344-4a25-bde9-a93ea14cb5fa
relation.isAuthorOfPublicationa7e94363-2dfa-4005-bcdc-2980bfbbd641
relation.isAuthorOfPublication.latestForDiscovery3224fce1-4344-4a25-bde9-a93ea14cb5fa

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