Publication:
Optimization of culture medium for the production and partial purification and characterization of an antibacterial activity from brevibacillus laterosporus strain EA62

dc.contributor.authorUsta, Alev A. K.
dc.contributor.authorDemirkan, Elif
dc.contributor.authorCengiz, Murat
dc.contributor.authorSevgi, Tuba
dc.contributor.authorZeren, Behice
dc.contributor.authorAbdou, Maoulida
dc.contributor.buuauthorUsta, Alev A. K.
dc.contributor.buuauthorDEMİRKAN, ELİF
dc.contributor.buuauthorCENGİZ, MURAT
dc.contributor.buuauthorSEVGİ, TUBA
dc.contributor.buuauthorZeren, Behice
dc.contributor.buuauthorAbdou, Maoulida
dc.contributor.departmentBursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü.
dc.contributor.departmentBursa Uludağ Üniversitesi/Veteriner Fakültesi/Farmakoloji ve Toksikoloji Anabilim Dalı.
dc.contributor.orcid0000-0002-5292-9482
dc.contributor.researcheridABI-4472-2020
dc.contributor.researcheridABE-5935-2020
dc.contributor.researcheridIPA-7484-2023
dc.contributor.researcheridISY-3462-2023
dc.contributor.researcheridEID-6407-2022
dc.contributor.researcheridCAU-1487-2022
dc.date.accessioned2024-09-25T11:59:45Z
dc.date.available2024-09-25T11:59:45Z
dc.date.issued2019-07-01
dc.description.abstractThe present study was performed to report the bacterial identification, optimization of culture conditions and characterization of a novel antibiotic substance from a Bacillus sp. EA62 strain isolated from soil. The EA62 strain was identified based on 16S rRNA analysis. The new isolate EBD 9-1 showed 100% sequence identity with Brevibacillus laterosporus. The antibacterial activity of the new substance was examined against five pathogenic bacteria and was observed to be the most effective against Escherichia coli. An agar diffusion assay was performed to evaluate the antibacterial activity of the substance. The effects of some nutritional (amino acid, carbon, nitrogen and metal sources) and physical factors (pH and temperature) and incubation time on the antibiotic activity were studied. Antibiotic activity in basal medium reached the maximum levels after 72 h of incubation. The best antibiotic activity was obtained in the presence of glucose as a carbon source, yeast extract as a nitrogen source, glutamic acid as an amino acid source and MgSO4+CaCO3 as metal ion sources. For physical parameters, the best results were obtained at 37 degrees C, pH 7.0. The antibiotic substance was partially purified, and the estimated molecular weight was 6.3 kDa. The minimum inhibitory concentration (MIC) values determined against five pathogen bacteria were >256 mu g/ml. The substance was identified by thin-layer chromatography, and its Rf value was measured as 0.04 cm.
dc.identifier.doi10.25083/rbl/24.4/705.713
dc.identifier.endpage713
dc.identifier.issn1224-5984
dc.identifier.issue4
dc.identifier.startpage705
dc.identifier.urihttps://doi.org/10.25083/rbl/24.4/705.713
dc.identifier.urihttps://www.e-repository.org/rbl/vol.24/iss.4/17.pdf
dc.identifier.urihttps://hdl.handle.net/11452/45243
dc.identifier.volume24
dc.identifier.wos000482118200017
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherArs Docendi
dc.relation.journalRomanian Biotechnological Letters
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBacillus-subtilis
dc.subjectAntimicrobial activity
dc.subjectSecondary metabolites
dc.subjectNitrogen-sources
dc.subjectCarbon
dc.subjectAlignment
dc.subjectPeptide
dc.subjectBrevibacillus
dc.subjectAntimicrobial substance
dc.subjectOptimization
dc.subjectPartial purification
dc.subjectBiotechnology & applied microbiology
dc.titleOptimization of culture medium for the production and partial purification and characterization of an antibacterial activity from brevibacillus laterosporus strain EA62
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication13b5deac-f120-4c0e-a42a-e8a47c700511
relation.isAuthorOfPublicationa96ca6a7-d471-4c71-a72f-1648cc98f911
relation.isAuthorOfPublicationef63b084-8975-4ca3-99a2-96753a035743
relation.isAuthorOfPublication.latestForDiscovery13b5deac-f120-4c0e-a42a-e8a47c700511

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