Publication:
Optimization and validation of ultrasensitive GC-MS/MS method to measure oxidatively induced DNA damage products and role of antioxidants in oxidation mechanism

dc.contributor.authorAybastıer, Önder
dc.contributor.authorDemir, Cevdet
dc.contributor.buuauthorAYBASTIER, ÖNDER
dc.contributor.buuauthorDEMİR, CEVDET
dc.contributor.departmentBursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.
dc.contributor.orcid0000-0002-0380-1992
dc.contributor.orcid0000-0002-9381-0410
dc.contributor.researcheridX-4621-2018
dc.contributor.researcheridDTQ-5753-2022
dc.date.accessioned2024-06-11T05:56:58Z
dc.date.available2024-06-11T05:56:58Z
dc.date.issued2021-04-08
dc.description.abstractOxidation of DNA due to exposure to reactive oxygen species (ROS) is a major source of DNA damage. ROS induced damage to DNA plays an important role in some diseases such as various cancers, aging and neurodegenerative diseases. The detection of DNA oxidation products plays a major role in assessing the mutagenicity potential of specific exposure. The GC-MS/MS method was developed for the ultrasensitive determination of individual DNA damage products. The validation results revealed that the proposed method was reliable and sensitive. Multiple response surface methodology (MRSM) was used to optimize derivatization conditions of oxidatively DNA base damage products before GC-MS/MS analysis. The optimum derivatization conditions were determined as 40 min for derivatization time, 120 degrees C for derivatization temperature and 1.4 for BSTFA/pyridine ratio under nitrogen atmosphere. The effects of thymol, carvacrol and thymoquinone as antioxidants were investigated on oxidative DNA damage. The determination of the oxidatively induced DNA damage products was performed after adding DNA and antioxidants with different concentrations under oxidative stress. Eighteen DNA base damage products were analyzed simultaneously using GC-MS/MS. This study showed a significant decrease in the amount of DNA base damage products when the antioxidants were present in the medium.
dc.identifier.doi10.1016/j.jpba.2021.114068
dc.identifier.eissn1873-264X
dc.identifier.issn0731-7085
dc.identifier.urihttps://doi.org/10.1016/j.jpba.2021.114068
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0731708521001795?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/11452/41953
dc.identifier.volume200
dc.identifier.wos000649654900005
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherElsevier
dc.relation.bap2014/5
dc.relation.journalJournal of Pharmaceutical and Biomedical Analysis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChromatography mass-spectrometry
dc.subjectRepair
dc.subjectCarvacrol
dc.subjectThymol
dc.subjectTool
dc.subjectDna damage
dc.subjectGC-MS/MS
dc.subjectDerivatization
dc.subjectValidation
dc.subjectMultiple response surface methodology
dc.subjectOxidative stress
dc.subjectChemistry
dc.subjectPharmacology & pharmacy
dc.titleOptimization and validation of ultrasensitive GC-MS/MS method to measure oxidatively induced DNA damage products and role of antioxidants in oxidation mechanism
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication875c0dc3-9c4b-47a6-8052-c7f44f84fc9b
relation.isAuthorOfPublication69347f0b-c785-46ac-ad11-78021d78b133
relation.isAuthorOfPublication.latestForDiscovery875c0dc3-9c4b-47a6-8052-c7f44f84fc9b

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Aybastıer_ve_Demir_2021.pdf
Size:
2.11 MB
Format:
Adobe Portable Document Format

Collections