Publication:
Determination of carotenoids in Chlamydomonas reinhardth by multivariate analysis with chromatographic data

dc.contributor.authorŞahin, Saliha
dc.contributor.authorYüksel, Çiğdem
dc.contributor.buuauthorŞAHİN, SALİHA
dc.contributor.buuauthorYüksel, Çiğdem
dc.contributor.departmentBursa Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Kimya Bölümü.
dc.contributor.researcheridAAH-2892-2021
dc.contributor.researcheridKHL-4329-2024
dc.date.accessioned2024-10-07T12:07:10Z
dc.date.available2024-10-07T12:07:10Z
dc.date.issued2019-01-01
dc.description.abstractThe carotenoid content of microalgae is an important quality parameter for nutritional and biological value. Determination of carotenoid content by spectroscopic and chromatographic methods is expensive and time-consuming. This study deals with the determination of carotenoids (0-carotene, zeaxanthin, neoxanthin, lutein and a-carotene) in Chlamydomonas reinhardtii by chromatography combination with principal component regression (PCR) and partial least squares (PLS) methods. Although, the chromatographic peaks of zeaxanthin and lutein were not separated by high performance liquid chromatography, PCR and PLS methods were employed successfully for the prediction of carotenoids in Chlamydomonas reinhardtii. It was found that the root mean square errors of prediction (RMSEP) for the validation set (r12=0) of PLS of 0-carotene, zeaxanthin, neoxanthin, lutein and a-carotene was 0.0500, 0.4300, 0.0252, 0.2800, and 0.1320, respectively. PLS method was used to improve resolution and quantitative determination of carotenoid overlapped highly in complicated natural samples.
dc.identifier.endpage4915
dc.identifier.issn1018-4619
dc.identifier.issue6
dc.identifier.startpage4909
dc.identifier.urihttps://hdl.handle.net/11452/45994
dc.identifier.volume28
dc.identifier.wos000473562700064
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherParlar Scientific Publications (P S P)
dc.relation.bapOUAP (F)- 2013/12
dc.relation.journalFresenius Environmental Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectPartial least-squares
dc.subjectCalibration
dc.subjectRegression
dc.subjectExtraction
dc.subjectDyes
dc.subjectPls
dc.subjectPcr
dc.subjectCarotenoid
dc.subjectHplc
dc.subjectMicroalgae
dc.subjectEnvironmental sciences & ecology
dc.titleDetermination of carotenoids in Chlamydomonas reinhardth by multivariate analysis with chromatographic data
dc.typeArticle
dspace.entity.typePublication
relation.isAuthorOfPublication4b87bb21-e6b9-4caf-a469-4b4fad49f199
relation.isAuthorOfPublication.latestForDiscovery4b87bb21-e6b9-4caf-a469-4b4fad49f199

Files

Collections