Molecularly imprinted surface plasmon resonance (SPR) sensor for uric acid determination
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Date
2017-11
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Elsevier
Abstract
The main objective of this study was to prepare a surface plasmon resonance (SPR) sensor for uric acid (UA) detection by using molecularly imprinted nanoparticles as a molecular recognition element. For imprinting, metal ion mediated preorganization was performed to interact between template molecules (UA) and functional monomer by using Fe3+ ions. UA-imprinted poly (hydroxyethyl methacrylate methacryloyl-l-cysteine methyl ester)-Fe3+ [poly(HEMA-MAC)-Fe3+] nanoparticles were synthesized by emulsion polymerization in the presence of MAC-Fe3+-UA pre-polymerization complex. The characterization of UA-imprinted poly(HEMA-MAC)-Fe3+ nanoparticles was conducted by Fourier transform infrared spectroscopy (FTIR), elemental analysis, scanning electron microscopy (SEM), energy dispersive x-ray spectroscopy (EDS), transmission electron microscopy (TEM) and zeta size analysis. The SPR sensor was prepared by gold surface modification of the sensor with UA-imprinted poly(HEMA-MAC)-Fe3+ nanoparticles. Characterization of the SPR sensor surface was performed with atomic force microscopy (AFM), contact angle (CA) and optic profilometer measurements. UA sensing ability of the prepared sensor was determined by interacting UA solutions in different concentrations (0.5-40 mg/L) with the SPR sensor. The limit of detection (LOD) and limit of quantification (LOQ) values for the prepared SPR sensor were calculated as 0.247 and 0.825 mg/L for aqueous solution, respectively. The UA-imprinted sensor was also used for UA detection in urine. The results showed the SPR sensor has high selectivity and sensitivity for UA.
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Keywords
Chemistry, Electrochemistry, Instruments & instrumentation, Metal ion imprinting, Molecular imprinting, Nanoparticle, SPR sensor, Uric acid, Carbon-paste electrodes, Amperometric determination, Electrochemical detection, Liquid-chromatograph, Ascorbic-acid, Glassy-carbon, Membrane, Amino acids, Atomic force microscopy, Electron microscopy, Emulsification, Emulsion polymerization, Energy dispersive spectroscopy, Fourier transform infrared spectroscopy, High resolution transmission electron microscopy, Iron compounds, Metal ions, Metal nanoparticles, Organic acids, Organic polymers, Plasmons, Scanning electron microscopy, Solutions, Surface treatment, Synthesis (chemical), Transmission electron microscopy, X ray spectroscopy, Energy dispersive X ray spectroscopy, Metal-ion imprintings, Molecular imprinting, Molecular recognition element, Pre-polymerization complex, SPR sensors, Surface plasmon resonance sensor, Uric acids, Surface plasmon resonance, Voltammetric method, Biosensor, Spectrometry
Citation
Sarıkaya, A. G. vd. (2017). ''Molecularly imprinted surface plasmon resonance (SPR) sensor for uric acid determination''. Sensors and Actuators, B: Chemical, 251, 763-772.