Microcontact imprinted surface plasmon resonance sensor for myoglobin detection
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Date
2013-10-01
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Elsevier
Abstract
In this study, we prepared surface plasmon resonance (SPR) sensor using the molecular imprinting technique for myoglobin detection in human serum. For this purpose, we synthesized myoglobin imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-t-tryptophan methyl ester) [poly(HEMA-MATrp)] nanofilm on the surface of SPR sensor. We also synthesized non-imprinted poly(HEMA-MATrp) nanofilm without myoglobin for the control experiments. The SPR sensor was characterized with contact angle measurements, atomic force microscopy, X-ray photoelectron spectroscopy, and ellipsometry. We investigated the effectiveness of the sensor using the SPR system. We evaluated the ability of SPR sensor to sense myoglobin with myoglobin solutions (pH 7.4, phosphate buffer) in different concentration range and in the serum taken from a patient with acute myocardial infarction. We found that the Langmuir adsorption model was the most suitable for the sensor system. The detection limit was 87.6 ng/mL. In order to show the selectivity of the SPR sensor, we investigated the competitive detection of myoglobin, lysozyme, cytochrome c and bovine serum albumin. The results showed that the SPR sensor has high selectivity and sensitivity for myoglobin.
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Keywords
Materials science, Molecularly imprinted polymer, Microcontact imprinting, SPR sensor, Myoglobin, Nanofilm, Myocardial-infarction, Protein recognition, Ribonuclease-a, Polymers, Lysozyme, Nanoparticles, Composite, Biosensor, Diagnosis, Cryogels, Amino acids, Atomic force microscopy, Body fluids, Photoelectrons, Plasmons, Sensors, X ray photoelectron spectroscopy, Microcontact imprinting, Molecularly imprinted polymer, Myoglobin, Nanofilm, SPR sensor, Micro-contact imprinting, Molecularly imprinted polymer, Myoglobin, Nano films, SPR sensors, Surface plasmon resonance
Citation
Osman, B. vd. (2013). "Microcontact imprinted surface plasmon resonance sensor for myoglobin detection". Materials Science and Engineering C-Metarials for Biological Applications, 33(7), 3609-3614.