Person: ÜSTÜNER, BURCU
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ÜSTÜNER
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BURCU
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Publication Does rainbow trout seminal plasma-cysteine combination affect the cryo-survivability and post-thaw incubation resilience of ram semen?(Mary Ann Liebert, Inc, 2022-12-13) Gökce, Elif; Üstüner, Burcu; ÜSTÜNER, BURCU; Veteriner Fakültesi; AAG-7238-2021The aim of this study was to evaluate the effect of both pure rainbow trout seminal plasma (RTSP) supplementation and RTSP-cysteine combination on cryopreservation success and post-thaw incubation resilience of ram semen in the nonbreeding season. For this purpose, different doses of RTSP (0%, 1%, 10%, and 15%) with or without cysteine supplementation were used for experiments. Ejaculates chosen for experiments were pooled and then divided into eight equal volumes for grouping (Control-ControlC, RTSP1-RTSP1C, RTSP10-RTSP10C, and RTSP15-RTSP15C). After cryopreservation, frozen-thawed semen samples were incubated for 5 hours at 37 degrees C for determination of post-thaw incubation resistance. Motility, HOST, TUNEL, Rh123-PI, and CTC tests were performed at 0 hour and 3rd and 5th hours of post-thaw incubation to evaluate the efficacy of all experimental groups. The RTSP10 and RTSP10C groups were noted to provide the best protection on motility, plasma membrane integrity, DNA integrity, and mitochondrial function of cryopreserved ram semen. On the other hand, the best protection against cryo-capacitation was observed in RTSP15 and RTSP15C groups. The addition of cysteine was found to be effective when the higher (15%) or lower (1%) doses of RTSP were used, as well as for no use of RTSP.Publication Lyophilized extender supplemented with rainbow trout (oncorhynchus mykiss) seminal plasma improves cryopreservation of ram sperm(Kafkas Üniversitesi, 2022-03-13) Üstuner, Burcu; Alçay, Selim; Gökçe, Elif; Yılmaz, M. Melih; Aktar, Ahmet; Hüraydın, Oğuzhan; Duman, Muhammed; Önder, N. Tekin; Akal, Eser; Nur, Zekariya; ÜSTÜNER, BURCU; ALÇAY, SELİM; Yılmaz, M. Melih; Aktar, Ahmet; Hüraydın, Oğuzhan; DUMAN, MUHAMMED; NUR, ZEKARİYA; Veteriner Fakültesi; Su Hayvanları Hastalıkları Ana Bilim Dalı; 0000-0002-1438-221X ; 0000-0001-7707-2705 ; 0000-0002-4125-6841 ; AAG-7238-2021; CBC-7350-2022; GWC-2055-2022; CBC-1744-2022; AHB-0614-2022; T-1697-2019; AAH-2635-2021This study aimed to investigate the effect of the Rainbow trout seminal plasma (RTSP) supplemented (control, 10% or 15%) lyophilized extender on freezability of ram semen. Collected semen was pooled and split into two aliquots, and each of the ejaculates was diluted with fresh or lyophilized extenders with RTSP (0%, FC and LC; 10% F10 and L10or 15% F15 and L15) using two-step dilution method. Semen was frozen using the programmable freezing machine. Semen samples were examined for sperm motility, defective acrosomes, plasma membrane integrity and DNA fragmentation at native and post-thaw stage. The highest percentages of post-thaw motility and plasma integrity were observed in the F10, F15, and L15 (P 0.05). There was no significant difference in the rate of post-thaw defective acrosomes when the other extender groups were compared with the control (P 0.05). The highest percentage of post-thaw DNA fragmentation values were observed in the FC group, while the lowest DNA fragmentation was obtained in the F15 but only significant different from FC and LC groups. In conclusion, the findings of this study show that the lyophilized extender with 15% RTSP added can be used successfully for freezing ram semen.Publication Drone semen cryopreservation with protein supplemented tl-hepes based extender(Kafkas Univ, Veteriner Fakultesi Dergisi, 2019-07-01) Alcay, Selim; ALÇAY, SELİM; Çakmak, Selvinar; Çakmak, İbrahim; ÇAKMAK, İBRAHİM; Mülkpınar, Emine; Toker, Mehmed Berk; TOKER, MEHMED BERK; Üstüner, Burcu; ÜSTÜNER, BURCU; Şen, Hasan; Nur, Zekariya; NUR, ZEKARİYA; Veteriner Fakültesi; 0000-0002-1438-221X; AAH-2635-2021; AAH-2558-2021; AAG-7238-2021; A-2794-2014The aim of the current study was to determine the optimum concentration of bovine serum albumin for post-thawing quality of drone sperm and this is the first study to evaluate the effect of BSA supplemented TL-Hepes based extenders for drone semen cryopreservation. Sexually mature drones were used for semen collection. Pooled semen was diluted with TL-Hepes based extender supplemented with different concentrations of BSA (1 mg/mL, 3 mg/mL, and 5 mg/mL) or without BSA (control), at a final concentration of 100x10(6) spermatozoon/mL. Motility, plasma membrane functional integrity (HOST), and defected acrosome (PSA-FITC) were evaluated in the study. At post thaw, the highest sperm motility rates were obtained in the BSA5 group (P< 0.05). Functional integrity of sperm membrane was better preserved in the BSA3 and BSA5 groups compared to the other groups. The acrosomal integrity rates were higher in BSA5 group than in control group (P< 0.05). The study shows that bovine serum albumin supplemented TL-Hepes based extenders have beneficial effect on drone semen parameters at post-thaw. The results of the study demonstrated a notable advantage of using 5 mg/mL of BSA in TL-Hepes based extender.Publication L-Carnitine supplemented extendersimprove post-thawing quality of honey bee drone (Apis mellifera) Spermatozoa [1](Kafkas Üniversitesi, 2021-07-01) Alçay, Selim; Çakmak, Selvinar; Çakmak, İbrahim; Aktar, Ahmet; Yılmaz, Melih; Üstüner, Burcu; Akkaşoğlu, Mustafa; Taşkıran, Şeyma; Ayaz, Elif; Sağırkaya, Hakan; Nur, Zekariya; ALÇAY, SELİM; Çakmak, Selvinar; ÇAKMAK, İBRAHİM; Aktar, Ahmet; Yılmaz, Melih; ÜSTÜNER, BURCU; Akkaşoğlu, Mustafa; Taşkıran, Şeyma; Ayaz, Elif; SAĞIRKAYA, HAKAN; NUR, ZEKARİYA; Arıcılığı Geliştirme Uygulama ve Araştırma Merkezi; Döreme ve Suni Tohumlama Ana Bilim Dalı; 0000-0002-8000-5770; 0000-0002-2975-2594; 0000-0002-1438-221X; CBC-7350-2022; ENZ-0848-2022; LMK-3522-2024; CBC-1744-2022; HSP-0413-2023; AAG-7238-2021; EMD-6376-2022; EAY-3244-2022; IBA-3364-2023; DPO-0081-2022; AAH-2635-2021The study aimed to evaluate the effects of L-carnitine on honey bee (Apis mellifera) drone spermatozoon at post-thaw. Semen samples obtained from sexually mature drones were pooled. Then, pooled semen was diluted with different concentrations of L-carnitine (2.5 mM/5 mM/10 mM) and without L-carnitine (control) supplemented extenders. Motility, plasma membrane functional integrity, acrosomal integrity and mitochondrial function were negatively affected by the cryopreservation process but DNA integrity was not affected. Malondialdehyde (MDA) concentration was used for analyzing the membrane lipid peroxidation status and a better result was obtained in the LC5 group compared with the control group at post-thaw. L-carnitine groups had a positive effect on sperm motility, plasma membrane integrity, and mitochondrial function compared to the control group (P<0.05). Acrosomal integrity was better preserved in the LC5 group compared to the control group. The study shows that LC supplemented extenders have beneficial effects on honey bee drone sperm parameters. The results of the present study demonstrated the beneficial effect of using a 5 mMol LC supplemented extender.Publication Effects of triton x-100 pretreatment of lyophilized and frozen-thawed ram sperm on preimplantation embryo developmental competence(Taylor & Francis Inc, 2022-02-19) Üstüner, Burcu; Yağcıoglu, Selin; Demir, Kamber; Gökce, Elif; Öztürk, Gül Bakirer; Birler, Sema; Pabuçcuoğlu, Serhat; Üstüner, Burcu; SAĞIRKAYA, HAKAN; ÜSTÜNER, BURCU; Sağırkaya, Hakan; Nur, Zekariya; NUR, ZEKARİYA; Alcay, Selim; ALÇAY, SELİM; Toker, Berk; TOKER, MEHMED BERK; Soylu, Mustafa Kemal; SOYLU, MUSTAFA KEMAL; Veteriner Fakültesi; Dölerme ve Suni Tohumlama Ana Bilim Dalı; 0000-0002-1438-221X; 0000-0003-4033-9749; 0000-0002-4690-1029; ACB-3704-2022In this study, it was aimed to determine the effect of destruction of lyophilized and frozen-thawed ram sperm plasma and acrosomal membrane on development of embryos produced by intracytoplasmic sperm injection (ICSI). Semen samples were divided into two groups for lyophilization (L) and freezing (F). For the removal of the plasma membrane, L and F groups were incubated with Triton X-100 (LTX-100 and FTX-100, respectively). Integrities of the plasma membrane, acrosome and chromatin structure were evaluated. Oocytes were injected with these sperm groups. Although no plasma membrane and acrosome integrities of the L (0.0%) group were detected, the plasma membrane integrity of the F group (69.4%) was significantly higher than the FTX-100 group (23.6%) (p < 0.05). The acrosome integrity of the FTX-100 group (3.80%) was significantly lower than the F group (55.6%) (p < 0.05). The chromatin integrities of L and F groups were higher than the Triton X-100 treated groups (p < 0.05). ICSIs with L, LTX-100, F and FTX-100 sperm were produced similar cleavage and blastocyst rates. In conclusion, data presented here confirm that ram spermatozoa can effectively be lyophilized and injected into oocytes for initiation of embryonic development and Triton X-100 pretreatment is not necessary while using lyophilized and frozen semen.Publication The effect of concentration and storage time on short-term storage of ram sperm(Kafkas Univ, Veteriner Fakultesi Dergisi, 2022-11-19) Dayanıklı, Cemal; Şengül, Ebru; Bülbül, Bülent; Nur, Zekariya; ÜSTÜNER, BURCU; NUR, ZEKARİYA; Veteriner Fakültesi; 0000-0002-1438-221X; AAH-2635-2021The present study was conducted to observe the effect of different dilution rates on ram sperm motion characters (motility, progressivity, velocity and average of speed), head diameter, mitochondrial membrane potential (MMP) and live intact acrosome rate during liquid storage for 96 h at 5 degrees C. Normospermic semen samples that obtained from 4 adult rams were pooled. Pooled semen was split into two aliquots; Low Dilution Rate (LDR) (400 million) and High Dilution Rate ( HDR) (20 million) motile spermatozoa per insemination dose/0.25 mL straw and were diluted with a skim milk-egg yolk-based extender. It was observed that the Total Motility (TM) and total progressivity of the LDR group decreased more rapidly than HDR group over time, in general. From the 12th h to the 96th h; the rapid progressivity (P<0.01), MMP (P<0.05), live sperm rates with intact acrosome (P<0.01) and straightness (STR) (P<0.001) of the HDR group results were statistically better than LDR. It was concluded that the spermatological properties examined during storage were damaged at different times and were affected by sperm concentration and also storage time.Publication Effects of various cryoprotective agents on post-thaw drone semen quality(Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 2015-01-01) Alçay, Selim; Üstüner, Burcu; Çakmak, İbrahim; Çakmak, Selvinar; Nur, Zekariya; ALÇAY, SELİM; ÜSTÜNER, BURCU; ÇAKMAK, İBRAHİM; Çakmak, Selvinar; NUR, ZEKARİYA; Veteriner Fakültesi; Tohumlama Ana Bilim Dalı; 0000-0002-1438-221X; AAH-2558-2021; AAG-7238-2021; AAH-2635-2021; JJQ-9411-2023; JLJ-9258-2023The aim of the present study was to evaluate the effect of different cryoprotectants on post-thaw semen motility and plasma membrane integrity of drone spermatozoa. Semen was obtained from mature drones (16 days or older) harvested from four colonies. Collected semen was diluted to a final concentration of 1/5 (semen/extender) in 0% cryoprotectant (control), 6% glycerol, 6% Ethylene Glycol, 6% 1,2 propanediol or 6% DMSO using a two-step dilution method. The equilibrated semen was frozen in 0.25-ml straws. The percentage of sperm motility and swollen tails (HOST) spermatozoa were evaluated following dilution with extender A (non-cryoprotectant), equilibration and post-thaw stages. In terms of post-thaw motility and plasma membrane integrity recovery we can rank the used cryoprotectant as DMSO, Ethylene Glycol, Glycerol and 1,2 Propanediol; respectively. In conclusion, post-thaw sperm motility and plasma membrane integrity of the present study was significantly better when sperm was frozen in DMSO with respect to control, glycerol, ethylene glycol, 1,2 propanediol (P<0.05).