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ÜSTÜNER, BURCU

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ÜSTÜNER

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    Publication
    Drone semen cryopreservation with protein supplemented tl-hepes based extender
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2019-07-01) Alcay, Selim; ALÇAY, SELİM; Çakmak, Selvinar; Çakmak, İbrahim; ÇAKMAK, İBRAHİM; Mülkpınar, Emine; Toker, Mehmed Berk; TOKER, MEHMED BERK; Üstüner, Burcu; ÜSTÜNER, BURCU; Şen, Hasan; Nur, Zekariya; NUR, ZEKARİYA; Bursa Uludağ Üniversitesi/Veteriner Fakültesi; 0000-0002-1438-221X; AAH-2635-2021; AAH-2558-2021; AAG-7238-2021; A-2794-2014
    The aim of the current study was to determine the optimum concentration of bovine serum albumin for post-thawing quality of drone sperm and this is the first study to evaluate the effect of BSA supplemented TL-Hepes based extenders for drone semen cryopreservation. Sexually mature drones were used for semen collection. Pooled semen was diluted with TL-Hepes based extender supplemented with different concentrations of BSA (1 mg/mL, 3 mg/mL, and 5 mg/mL) or without BSA (control), at a final concentration of 100x10(6) spermatozoon/mL. Motility, plasma membrane functional integrity (HOST), and defected acrosome (PSA-FITC) were evaluated in the study. At post thaw, the highest sperm motility rates were obtained in the BSA5 group (P< 0.05). Functional integrity of sperm membrane was better preserved in the BSA3 and BSA5 groups compared to the other groups. The acrosomal integrity rates were higher in BSA5 group than in control group (P< 0.05). The study shows that bovine serum albumin supplemented TL-Hepes based extenders have beneficial effect on drone semen parameters at post-thaw. The results of the study demonstrated a notable advantage of using 5 mg/mL of BSA in TL-Hepes based extender.
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    Publication
    Effects of various cryoprotective agents on post-thaw drone semen quality
    (Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 2015-01-01) Alçay, Selim; Üstüner, Burcu; Çakmak, İbrahim; Çakmak, Selvinar; Nur, Zekariya; ALÇAY, SELİM; ÜSTÜNER, BURCU; ÇAKMAK, İBRAHİM; Çakmak, Selvinar; NUR, ZEKARİYA; Uludağ Üniversitesi/Veteriner Fakültesi/Tohumlama Anabilim Dalı; Uludağ Üniversitesi/Veteriner Fakültesi/Arıcılık Geliştirme Uygulama ve Araştırma Merkezi; 0000-0002-1438-221X; AAH-2558-2021; AAG-7238-2021; AAH-2635-2021; JJQ-9411-2023; JLJ-9258-2023
    The aim of the present study was to evaluate the effect of different cryoprotectants on post-thaw semen motility and plasma membrane integrity of drone spermatozoa. Semen was obtained from mature drones (16 days or older) harvested from four colonies. Collected semen was diluted to a final concentration of 1/5 (semen/extender) in 0% cryoprotectant (control), 6% glycerol, 6% Ethylene Glycol, 6% 1,2 propanediol or 6% DMSO using a two-step dilution method. The equilibrated semen was frozen in 0.25-ml straws. The percentage of sperm motility and swollen tails (HOST) spermatozoa were evaluated following dilution with extender A (non-cryoprotectant), equilibration and post-thaw stages. In terms of post-thaw motility and plasma membrane integrity recovery we can rank the used cryoprotectant as DMSO, Ethylene Glycol, Glycerol and 1,2 Propanediol; respectively. In conclusion, post-thaw sperm motility and plasma membrane integrity of the present study was significantly better when sperm was frozen in DMSO with respect to control, glycerol, ethylene glycol, 1,2 propanediol (P<0.05).