Publication: Effects of various cryoprotective agents on post-thaw drone semen quality
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Date
2015-01-01
Authors
Authors
Alçay, Selim
Üstüner, Burcu
Çakmak, İbrahim
Çakmak, Selvinar
Nur, Zekariya
Journal Title
Journal ISSN
Volume Title
Publisher
Kafkas Üniversitesi Veteriner Fakültesi Dergisi
Abstract
The aim of the present study was to evaluate the effect of different cryoprotectants on post-thaw semen motility and plasma membrane integrity of drone spermatozoa. Semen was obtained from mature drones (16 days or older) harvested from four colonies. Collected semen was diluted to a final concentration of 1/5 (semen/extender) in 0% cryoprotectant (control), 6% glycerol, 6% Ethylene Glycol, 6% 1,2 propanediol or 6% DMSO using a two-step dilution method. The equilibrated semen was frozen in 0.25-ml straws. The percentage of sperm motility and swollen tails (HOST) spermatozoa were evaluated following dilution with extender A (non-cryoprotectant), equilibration and post-thaw stages. In terms of post-thaw motility and plasma membrane integrity recovery we can rank the used cryoprotectant as DMSO, Ethylene Glycol, Glycerol and 1,2 Propanediol; respectively. In conclusion, post-thaw sperm motility and plasma membrane integrity of the present study was significantly better when sperm was frozen in DMSO with respect to control, glycerol, ethylene glycol, 1,2 propanediol (P<0.05).
Description
Keywords
Honey-bee queens, Cryopreservation, Hymenoptera, Extender, Drone spermatozoa, Cryoprotectants, Cryopreservation, Science & technology, Life sciences & biomedicine, Veterinary sciences