Publication: Effects of various cryoprotective agents on post-thaw drone semen quality
dc.contributor.author | Alçay, Selim | |
dc.contributor.author | Üstüner, Burcu | |
dc.contributor.author | Çakmak, İbrahim | |
dc.contributor.author | Çakmak, Selvinar | |
dc.contributor.author | Nur, Zekariya | |
dc.contributor.buuauthor | ALÇAY, SELİM | |
dc.contributor.buuauthor | ÜSTÜNER, BURCU | |
dc.contributor.buuauthor | ÇAKMAK, İBRAHİM | |
dc.contributor.buuauthor | Çakmak, Selvinar | |
dc.contributor.buuauthor | NUR, ZEKARİYA | |
dc.contributor.department | Uludağ Üniversitesi/Veteriner Fakültesi/Tohumlama Anabilim Dalı | |
dc.contributor.department | Uludağ Üniversitesi/Veteriner Fakültesi/Arıcılık Geliştirme Uygulama ve Araştırma Merkezi | |
dc.contributor.orcid | 0000-0002-1438-221X | |
dc.contributor.researcherid | AAH-2558-2021 | |
dc.contributor.researcherid | AAG-7238-2021 | |
dc.contributor.researcherid | AAH-2635-2021 | |
dc.contributor.researcherid | JJQ-9411-2023 | |
dc.contributor.researcherid | JLJ-9258-2023 | |
dc.date.accessioned | 2024-08-13T10:46:42Z | |
dc.date.available | 2024-08-13T10:46:42Z | |
dc.date.issued | 2015-01-01 | |
dc.description.abstract | The aim of the present study was to evaluate the effect of different cryoprotectants on post-thaw semen motility and plasma membrane integrity of drone spermatozoa. Semen was obtained from mature drones (16 days or older) harvested from four colonies. Collected semen was diluted to a final concentration of 1/5 (semen/extender) in 0% cryoprotectant (control), 6% glycerol, 6% Ethylene Glycol, 6% 1,2 propanediol or 6% DMSO using a two-step dilution method. The equilibrated semen was frozen in 0.25-ml straws. The percentage of sperm motility and swollen tails (HOST) spermatozoa were evaluated following dilution with extender A (non-cryoprotectant), equilibration and post-thaw stages. In terms of post-thaw motility and plasma membrane integrity recovery we can rank the used cryoprotectant as DMSO, Ethylene Glycol, Glycerol and 1,2 Propanediol; respectively. In conclusion, post-thaw sperm motility and plasma membrane integrity of the present study was significantly better when sperm was frozen in DMSO with respect to control, glycerol, ethylene glycol, 1,2 propanediol (P<0.05). | |
dc.identifier.doi | 10.9775/kvfd.2014.11515 | |
dc.identifier.endpage | 35 | |
dc.identifier.issn | 1300-6045 | |
dc.identifier.issue | 1 | |
dc.identifier.startpage | 31 | |
dc.identifier.uri | https://doi.org/10.9775/kvfd.2014.11515 | |
dc.identifier.uri | https://hdl.handle.net/11452/43972 | |
dc.identifier.volume | 21 | |
dc.identifier.wos | 000349190200006 | |
dc.indexed.wos | WOS.SCI | |
dc.language.iso | en | |
dc.publisher | Kafkas Üniversitesi Veteriner Fakültesi Dergisi | |
dc.relation.journal | Kafkas Üniversitesi Veteriner Fakültesi Dergisi | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | Honey-bee queens | |
dc.subject | Cryopreservation | |
dc.subject | Hymenoptera | |
dc.subject | Extender | |
dc.subject | Drone spermatozoa | |
dc.subject | Cryoprotectants | |
dc.subject | Cryopreservation | |
dc.subject | Science & technology | |
dc.subject | Life sciences & biomedicine | |
dc.subject | Veterinary sciences | |
dc.title | Effects of various cryoprotective agents on post-thaw drone semen quality | |
dc.type | Article | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 2f32ebee-28b4-4e2e-8f35-bcaf3ecf5ff1 | |
relation.isAuthorOfPublication | b2e53cfa-8af8-44b0-a5a4-a531c86ec47a | |
relation.isAuthorOfPublication | 461bac4a-4e4c-4778-a600-06a649d076ca | |
relation.isAuthorOfPublication | 01c4e084-43f9-441e-a5df-7f5525becc3a | |
relation.isAuthorOfPublication.latestForDiscovery | 2f32ebee-28b4-4e2e-8f35-bcaf3ecf5ff1 |