Publication:
Effects of various cryoprotective agents on post-thaw drone semen quality

dc.contributor.authorAlçay, Selim
dc.contributor.authorÜstüner, Burcu
dc.contributor.authorÇakmak, İbrahim
dc.contributor.authorÇakmak, Selvinar
dc.contributor.authorNur, Zekariya
dc.contributor.buuauthorALÇAY, SELİM
dc.contributor.buuauthorÜSTÜNER, BURCU
dc.contributor.buuauthorÇAKMAK, İBRAHİM
dc.contributor.buuauthorÇakmak, Selvinar
dc.contributor.buuauthorNUR, ZEKARİYA
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/Tohumlama Anabilim Dalı
dc.contributor.departmentUludağ Üniversitesi/Veteriner Fakültesi/Arıcılık Geliştirme Uygulama ve Araştırma Merkezi
dc.contributor.orcid0000-0002-1438-221X
dc.contributor.researcheridAAH-2558-2021
dc.contributor.researcheridAAG-7238-2021
dc.contributor.researcheridAAH-2635-2021
dc.contributor.researcheridJJQ-9411-2023
dc.contributor.researcheridJLJ-9258-2023
dc.date.accessioned2024-08-13T10:46:42Z
dc.date.available2024-08-13T10:46:42Z
dc.date.issued2015-01-01
dc.description.abstractThe aim of the present study was to evaluate the effect of different cryoprotectants on post-thaw semen motility and plasma membrane integrity of drone spermatozoa. Semen was obtained from mature drones (16 days or older) harvested from four colonies. Collected semen was diluted to a final concentration of 1/5 (semen/extender) in 0% cryoprotectant (control), 6% glycerol, 6% Ethylene Glycol, 6% 1,2 propanediol or 6% DMSO using a two-step dilution method. The equilibrated semen was frozen in 0.25-ml straws. The percentage of sperm motility and swollen tails (HOST) spermatozoa were evaluated following dilution with extender A (non-cryoprotectant), equilibration and post-thaw stages. In terms of post-thaw motility and plasma membrane integrity recovery we can rank the used cryoprotectant as DMSO, Ethylene Glycol, Glycerol and 1,2 Propanediol; respectively. In conclusion, post-thaw sperm motility and plasma membrane integrity of the present study was significantly better when sperm was frozen in DMSO with respect to control, glycerol, ethylene glycol, 1,2 propanediol (P<0.05).
dc.identifier.doi10.9775/kvfd.2014.11515
dc.identifier.endpage35
dc.identifier.issn1300-6045
dc.identifier.issue1
dc.identifier.startpage31
dc.identifier.urihttps://doi.org/10.9775/kvfd.2014.11515
dc.identifier.urihttps://hdl.handle.net/11452/43972
dc.identifier.volume21
dc.identifier.wos000349190200006
dc.indexed.wosWOS.SCI
dc.language.isoen
dc.publisherKafkas Üniversitesi Veteriner Fakültesi Dergisi
dc.relation.journalKafkas Üniversitesi Veteriner Fakültesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHoney-bee queens
dc.subjectCryopreservation
dc.subjectHymenoptera
dc.subjectExtender
dc.subjectDrone spermatozoa
dc.subjectCryoprotectants
dc.subjectCryopreservation
dc.subjectScience & technology
dc.subjectLife sciences & biomedicine
dc.subjectVeterinary sciences
dc.titleEffects of various cryoprotective agents on post-thaw drone semen quality
dc.typeArticle
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery2f32ebee-28b4-4e2e-8f35-bcaf3ecf5ff1

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