Publication: Positive effect of autologous platelet rich plasma on saanen buck semen cryopreservation in non-breeding season
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Date
2021-12-01
Authors
Authors
Alçay, Selim
Aktar, Ahmet
Koca, Davut
Kılıç, Mehmet Ali
Akkaşoğlu, Mustafa
Sağırkaya, Hakan
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Journal ISSN
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Publisher
Academic Press Inc Elsevier Science
Abstract
The objective of the current study was to evaluate the effect of autologous platelet-rich plasma (PRP) addition into soybean lecithin based extender on buck semen at post-thaw. Semen samples were collected from eight Saanen buck, and each semen sample was split into four equal aliquots and diluted with different concentrations of PRP supplemented extenders [no PRP (control), 0.5 x 107/ml PRP, 1 x 107/ml PRP, or 2 x 107/ml PRP]. Motility, plasma membrane functional integrity, acrosome integrity, mitochondrial membrane potential, DNA integrity and malondialdehyde concentrations (MDA) were measured and analyzed at post-thaw. The results showed that 2 x 107/ml PRP group had a positive effect on motility (62.41 +/- 4.24), membrane functional integrity (71.11 +/- 2.90), mitochondrial membrane potential (69.70 +/- 1.99), DNA integrity (7.22 +/- 0.93) and MDA levels (2.56 +/- 0.73) at post-thaw (P < 0.05). The results of the study demonstrated that autologous PRP has a protective effect on cryopreservation of buck spermatozoa and the fertility effects are worthy of further study.
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Keywords
Egg-yolk, Soybean lecithin, Incubation resilience, Oxidative stress, Postthaw quality, Sperm, Antioxidants, Extenders, Frozen, Fertility, Platelet-rich plasma, Buck semen, Cryopreservation, Post-thaw, Science & technology, Life sciences & biomedicine, Biology, Physiology, Life sciences & biomedicine - other topics