Effect of different thawing procedures on the quality of bull semen

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Date

2003-07

Journal Title

Journal ISSN

Volume Title

Publisher

Ecole Nationale Veterinaire Toulouse

Abstract

The objective of this study was to evaluate effect of different thawing procedure to Hypo-Osmotic Swelling Test (HOST), motility and morphology of bull semen. Straw (0.25 ml) frozen semen from 4 Holstein bulls was used in this study. Post-thaw motility, morphology and hypo-osmotic swelling test (HOST) were carried out at 37degreesC for 30 sec, 50degreesC for 15 sec and 70degreesC for 5 sec. According to these protocols motility, defected acrosome, other morphological defects, total morphological defects, and swollen tail spermatozoa (HOST) values were 56.6 %, 56.7 %, 59.8 %; 3.7 %, 2.6 1.9 %; 10.7 %, 8.8 %, 5.6 %; 14.4 %, 11.5 %, 7.5 %; 37.1 %, 37.4 39.3 % respectively. Thawing protocol has affected all sperm characteristics (P < 0.05) except HOST. There was a high correlation between HOST values and motility (P < 0.01) but no correlation was observed between HOST and acrosome defects, other morphological defects and total morphological defects.

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Keywords

Veterinary sciences, Animalia, Bos taurus, Bull, Frozen semen, Thawing regime, Membrane integrity, Membrane functional integrity, Sperm membrane, Acrosomal integrity, Bovine spermatozoa, Hypoosmotic test, Ram spermatozoa, Frozen, Temperatures, Viability, Motility

Citation

Nur, Z. vd. (2003). “Effect of different thawing procedures on the quality of bull semen”. Revue De Medecine Veterinaire, 154(7), 487-490.